Reuter Gábor, Boldizsár Akos, Pankovics Péter
Regional Laboratory of Virology, ANTSZ Regional Institute of State Public Health Service, Pécs, Hungary.
Arch Virol. 2009;154(1):101-8. doi: 10.1007/s00705-008-0288-2. Epub 2008 Dec 19.
Kobuvirus is a new genus in the family Picornaviridae. Two species are currently known: Aichi virus (human kobuvirus) and Bovine kobuvirus (U-1). In this study, the complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus (Kobuvirus/swine/S-1-HUN/2007/Hungary, EU787450) were determined. The structure of the S-1-HUN genome, VPg-5'UTR-leader protein-structural proteins (VP0, VP3, VP1)-non-structural proteins (2A-2C, 3A-3D)-3'UTR-poly(A) tail, was found to be typical of picornavirus. The 8210-nucleotide (nt)-long RNA genome contains a large open reading frame (7467 nt) encoding a potential polyprotein precursor of 2488 amino acids (aa) that has 57/56% and 63/64% nt/aa identity with Aichi virus and U-1, respectively. The 5'UTR contains a hepacivirus/pestivirus-like internal ribosomal entry site (IRES type IV group-B-like) with conserved pseudoknot, II and IIIa-f domains. A tandem repeat (a 30-amino-acid-long motif) was detected in 2B. Thirty-nine (65%) of the 60 fecal samples from pigs under the age of 6 months at the tested farm were positive (the incidence was 90% under the age of 3 weeks). Porcine kobuvirus belongs to a potential new species-the third-in the genus Kobuvirus.
杯状病毒是小核糖核酸病毒科中的一个新属。目前已知两个种：爱知病毒（人杯状病毒）和牛杯状病毒（U-1）。在本研究中，测定了猪杯状病毒（杯状病毒/猪/S-1-HUN/2007/匈牙利，EU787450）的完整核苷酸和氨基酸序列以及基因结构。发现S-1-HUN基因组的结构，即VPg-5'非翻译区-前导蛋白-结构蛋白（VP0、VP3、VP1）-非结构蛋白（2A-2C、3A-3D）-3'非翻译区-聚腺苷酸尾，是小核糖核酸病毒的典型结构。8210个核苷酸（nt）长的RNA基因组包含一个大的开放阅读框（7467 nt），编码一个由2488个氨基酸（aa）组成的潜在多蛋白前体，该前体与爱知病毒和U-1的核苷酸/氨基酸同源性分别为57/56%和63/64%。5'非翻译区包含一个丙型肝炎病毒/瘟病毒样内部核糖体进入位点（IV型B组样IRES），具有保守的假结、II和IIIa-f结构域。在2B中检测到一个串联重复序列（一个30个氨基酸长的基序）。在受试农场，60份6月龄以下仔猪的粪便样本中有39份（65%）呈阳性（在龄3周以下时发病率为90%）。猪杯状病毒属于杯状病毒属中一个潜在的新种——第三个新种。
