Panagiotidou Christina, Bouloumpasi Elisavet, Irakli Maria, Chatzopoulou Paschalina
Hellenic Agricultural Organization-Dimitra, Institute of Plant Breeding and Genetic Resources, P.O. Box 60458, 57001 Thessaloniki, Greece.
Plants (Basel). 2024 Nov 2;13(21):3087. doi: 10.3390/plants13213087.
Nowadays, eco-friendly extraction techniques are often used to develop natural plant extracts for commercial use. In the current investigation, Greek oregano () phenolic extracts from different cultivated accessions were recovered employing ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE), and accelerated solvent extraction (ASE). The phytochemical profile of the oregano extracts, as determined by spectrophotometric and chromatographic techniques, as well as antioxidant activity (ABTS, DPPH, and FRAP assays), was used to compare the three extraction approaches. The results showed that oregano phenolic extracts obtained by MAE held the highest total phenolic content, total flavonoid content, and also a higher content of the main phenolic compounds identified, rosmarinic acid, salvianolic acid B and carvacrol, as determined by LC-MS analysis, followed by those extracted by UAE and ASE. All of the tested extracts exhibited relatively high antioxidant activities, especially the MAE extracts. Oregano extracts produced by various extraction techniques were subjected to a multivariate data analysis to highlight differences in phytochemical profiles, and their correlation to antioxidant activity. According to our findings, it was evident that MAE offers more efficient and effective extraction of bioactive compounds in terms of obtaining phytochemical-rich oregano extracts, with applications in the food and pharmaceutical industries.
如今，环保型提取技术常被用于开发用于商业用途的天然植物提取物。在当前的研究中，采用超声辅助提取（UAE）、微波辅助提取（MAE）和加速溶剂萃取（ASE）从不同栽培品种的希腊牛至中回收酚类提取物。通过分光光度法和色谱技术测定的牛至提取物的植物化学特征以及抗氧化活性（ABTS、DPPH和FRAP测定）被用于比较这三种提取方法。结果表明，通过MAE获得的牛至酚类提取物的总酚含量、总黄酮含量最高，并且通过液相色谱-质谱分析确定的主要酚类化合物迷迭香酸、丹酚酸B和香芹酚的含量也更高，其次是通过UAE和ASE提取的提取物。所有测试提取物均表现出相对较高的抗氧化活性，尤其是MAE提取物。对通过各种提取技术生产的牛至提取物进行多变量数据分析，以突出植物化学特征的差异及其与抗氧化活性的相关性。根据我们的研究结果，很明显，就获得富含植物化学物质的牛至提取物而言，MAE在提取生物活性化合物方面更高效、有效，可应用于食品和制药行业。
