Kakiuchi Nobuko, Atsumi Toshiyuki, Higuchi Mari, Kamikawa Shohei, Miyako Haruka, Wakita Yuriko, Ohtsuka Isao, Hayashi Shigeki, Hishida Atsuyuki, Kawahara Nobuo, Nishizawa Makoto, Yamagishi Takashi, Kadota Yuichi
Kyusyu University of Health and Welfare, Nobeoka, Miyazaki, 882-8508, Japan,
J Nat Med. 2015 Jan;69(1):154-64. doi: 10.1007/s11418-014-0867-3. Epub 2014 Sep 9.
Aconite tuber is a representative crude drug for warming the body internally in Japanese Kampo medicine and Chinese traditional medicine. The crude drug is used in major prescriptions for the aged. Varieties of Aconitum plants are distributed throughout the Japanese Islands, especially Hokkaido. With the aim of identifying the medicinal potential of Aconitum plants from Hokkaido, 107 specimens were collected from 36 sites in the summer of 2011 and 2012. Their nuclear DNA region, internal transcribed spacer (ITS), and aconitine alkaloid contents were analyzed. Phylogenic analysis of ITS by maximum parsimony analysis showed that the majority of the specimens were grouped into one cluster (cluster I), separated from the other cluster (cluster II) consisting of alpine specimens. The aconitine alkaloid content of the tuberous roots of 76 specimens showed 2 aspects-specimens from the same collection site showed similar aconitine alkaloid profiles, and cluster I specimens from different habitats showed various alkaloid profiles. Environmental pressure of each habitat is presumed to have caused the morphology and aconitine alkaloid profile of these genetically similar specimens to diversify.
附子是日本汉方医学和中国传统医学中一种代表性的温里药材。该药材用于老年人的主要方剂中。乌头属植物品种分布于日本列岛各地，尤其是北海道。为了确定北海道乌头属植物的药用潜力，2011年和2012年夏季从36个地点采集了107份标本。分析了它们的核DNA区域、内部转录间隔区（ITS）和乌头碱生物碱含量。通过最大简约分析对ITS进行系统发育分析表明，大多数标本聚为一个簇（簇I），与由高山标本组成的另一个簇（簇II）分开。76份标本块根的乌头碱生物碱含量呈现两个方面——来自同一采集地点的标本显示出相似的乌头碱生物碱谱，而来自不同栖息地的簇I标本显示出不同的生物碱谱。推测每个栖息地的环境压力导致了这些基因相似标本的形态和乌头碱生物碱谱多样化。
