Thorsen S U, Eising S, Mortensen H B, Skogstrand K, Pociot F, Johannesen J, Svensson J
Department of Peadiatrics, Herlev Hospital, University of Copenhagen, Herlev, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Scand J Immunol. 2014 Dec;80(6):452-61. doi: 10.1111/sji.12240.
The mechanisms by which antigen-specific T cells migrate to the islets of Langerhans in type 1 diabetes (T1D) are largely unknown. Chemokines attract immune cells to sites of inflammation. The aim was to elucidate the role of inflammatory chemokines in T1D at time of diagnosis. From a population-based registry of children diagnosed with T1D from 1997 to 2005, we studied five different inflammatory chemokines (CCL2, CCL3, CCL4, CCL5 and CXCL8). Four hundred and eighty-two cases and 479 sibling frequencies matched on age and sample year distribution were included. Patients showed lower levels of CCL4 compared to siblings, but this result was not significant after correction for multiple testing. CCL2, CCL3, CCL4 and CXCL8 levels were highest in the most recent cohorts (P < 0.01) in both patients and siblings. A significant seasonal variation - for most of the chemokines - was demonstrated with the highest level during the summer period in both patients and siblings. In addition, there was a significant inverse relationship between CCL4 levels and age. When comparing patients and siblings, remarkably few differences were identified, but interestingly chemokine levels varied with age, season and period for the entire study population. Such variations should be taken into account when studying chemokines in paediatric populations.
1型糖尿病（T1D）中抗原特异性T细胞迁移至胰岛的机制在很大程度上尚不清楚。趋化因子可将免疫细胞吸引至炎症部位。本研究旨在阐明诊断时炎症趋化因子在T1D中的作用。我们从1997年至2005年确诊为T1D的儿童的基于人群的登记资料中，研究了五种不同的炎症趋化因子（CCL2、CCL3、CCL4、CCL5和CXCL8）。纳入了482例病例以及479例年龄和样本年份分布相匹配的同胞对照。与同胞相比，患者的CCL4水平较低，但在进行多重检验校正后，该结果无统计学意义。在患者和同胞中，CCL2、CCL3、CCL4和CXCL8水平在最近的队列中最高（P < 0.01）。在患者和同胞中，大多数趋化因子都呈现出显著的季节性变化，夏季水平最高。此外，CCL4水平与年龄之间存在显著的负相关关系。在比较患者和同胞时，发现的差异非常少，但有趣的是，整个研究人群的趋化因子水平随年龄、季节和时期而变化。在儿科人群中研究趋化因子时应考虑到这些变化。
