Yan Pingyu, Xie Zixiong, Feng Kele, Qiu Xinyu, Zhang Lei, Zhang Hanguo
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Heilongjiang Academy of Forestry, Harbin, China.
Front Plant Sci. 2023 Jan 16;13:1079571. doi: 10.3389/fpls.2022.1079571. eCollection 2022.
Korean pine is a native tree species in Northeast China. In order to meet the needs of germplasm resource evaluation and molecular marker-assisted breeding of Korean pine, we collected Korean pine clones from 7 populations in Northeast China, analyzed the genetic diversity and genetic structure by SSR molecular marker technology and clustered them to revealed the inter- and intrapopulation differentiation characteristics of each clone. The fingerprint profiles of 161 Korean pine clones were also constructed. 77 alleles were detected for 11 markers, and 18 genotypes were identified on average for each marker. The PIC of the different markers ranged from 0.155-0.855, and the combination of PI and PIsibs for the 11 markers was 3.1 × 10 and 1.14 × 10, respectively. MANOVA showed that genetic variation existed mainly within populations, accounting for 98% of the total variation. The level of genetic differentiation among populations was low, with an average between populations of 11.036. Genetic diversity is lower in the Lushuihe population and higher in the Tieli population. The 161 Korean pine clones were divided into 4 or 7 populations, and the 7 populations were not clearly distinguished from each other, with only the Lushuihe population showing partial differentiation. There is no significant correlation between the genetic distance of Korean pine populations and the geographical distance of their superior tree sources. This result can provide recommendations for future Korean pine breeding programs. The combination of 11 markers could completely distinguish 161 clones and establish the fingerprint. Genetic diversity of Korean pine clones from the 7 populations was abundant, and the genetic distances of individuals and populations were evenly dispersed. The fingerprint map can be used for the identification of Korean pine clones.
红松是中国东北地区的乡土树种。为满足红松种质资源评价和分子标记辅助育种的需求，我们从中国东北地区7个种群收集了红松无性系，采用SSR分子标记技术分析其遗传多样性和遗传结构，并进行聚类以揭示各无性系的种群间和种群内分化特征。还构建了161个红松无性系的指纹图谱。11个标记共检测到77个等位基因，每个标记平均鉴定出18种基因型。不同标记的PIC范围为0.155 - 0.855，11个标记的PI和PIsibs组合分别为3.1×10和1.14×10。多变量方差分析表明，遗传变异主要存在于种群内，占总变异的98%。种群间的遗传分化水平较低，种群间平均为11.036。露水河种群的遗传多样性较低，铁力种群的遗传多样性较高。161个红松无性系被分为4个或7个种群，7个种群之间没有明显区分，只有露水河种群表现出部分分化。红松种群的遗传距离与其优良树木来源的地理距离之间没有显著相关性。这一结果可为未来红松育种计划提供参考。11个标记的组合能够完全区分161个无性系并建立指纹图谱。来自7个种群的红松无性系遗传多样性丰富，个体和种群的遗传距离均匀分布。指纹图谱可用于红松无性系的鉴定。
