Soni Dharmendra Kumar, Dubey Suresh Kumar
Environmental Microbiology Lab, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.
Mol Biol Rep. 2014 Dec;41(12):8219-29. doi: 10.1007/s11033-014-3724-2. Epub 2014 Sep 10.
The discrimination between Listeria monocytogenes and Listeria species has been detected. The 16S rRNA and hlyA were PCR amplified with set of oligonucleotide primers with flank 1,500 and 456 bp fragments, respectively. Based on the differences in 16S rRNA and hlyA genes, a total 80 isolates from different environmental, food and clinical samples confirmed it to be L. monocytogenes. The 16S rRNA sequence similarity suggested that the isolates were similar to the previously reported ones from different habitats by others. The phylogenetic interrelationships of the genus Listeria were investigated by sequencing of 16S rRNA and hlyA gene. The 16S rRNA sequence indicated that genus Listeria is comprised of following closely related but distinct lines of descent, one is the L. monocytogenes species group (including L. innocua, L. ivanovii, L. seeligeri and L. welshimeri) and other, the species L. grayi, L. rocourtiae and L. fleischmannii. The phylogenetic tree based on hlyA gene sequence clearly differentiates between the L. monocytogenes, L. ivanovii and L. seeligeri. In the present study, we identified 80 isolates of L. monocytogenes originating from different clinical, food and environmental samples based on 16S rRNA and hlyA gene sequence similarity.
已检测到单核细胞增生李斯特菌与李斯特菌属其他菌种之间的差异。使用一组寡核苷酸引物对16S rRNA和hlyA进行PCR扩增，分别扩增出侧翼为1500 bp和456 bp的片段。基于16S rRNA和hlyA基因的差异，从不同环境、食品和临床样本中总共分离出80株菌株，证实它们为单核细胞增生李斯特菌。16S rRNA序列相似性表明，这些分离株与其他人先前报道的来自不同栖息地的菌株相似。通过对16S rRNA和hlyA基因进行测序，研究了李斯特菌属的系统发育关系。16S rRNA序列表明，李斯特菌属由以下密切相关但不同的谱系组成，一个是单核细胞增生李斯特菌种组（包括无害李斯特菌、伊氏李斯特菌、西尔李斯特菌和威尔士李斯特菌），另一个是格氏李斯特菌、罗氏李斯特菌和弗莱施曼李斯特菌。基于hlyA基因序列的系统发育树清楚地区分了单核细胞增生李斯特菌、伊氏李斯特菌和西尔李斯特菌。在本研究中，我们基于16S rRNA和hlyA基因序列相似性，鉴定出80株源自不同临床、食品和环境样本的单核细胞增生李斯特菌分离株。
