Aksono Eduardus Bimo, Riwu Katty Hendriana Priscilla, Estoepangestie A T Soelih, Pertiwi Herinda
Department of Basic Veterinary Medicine, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Institute of Tropical Disease, Department of Health, Faculty of Vocational Studies, Airlangga University, Surabaya, Jawa Timur 60115, Indonesia.
Vet Med Int. 2020 Mar 1;2020:9761812. doi: 10.1155/2020/9761812. eCollection 2020.
The objective of this study was to identify the phylogenetic analysis and antibiotic resistance of contaminating chicken meat in Surabaya. 60 chicken meat samples were collected from supermarkets, mobile vendors, and traditional markets in Surabaya. A selective medium is used for isolation and identification of by chopping 25 grams of the chicken meat and to put it into the sterilized Erlenmeyer flasks. Some methods were used for the identification procedures, such as biochemical and morphological tests, antibiotic resistance test, PCR, and sequencing; also a phylogenetic analysis was conducted by a neighbor-joining analysis using Genetix Mac ver 8.0 with hlyA genes of recorded in GenBank, such as Lineage I (KC808543), Lineage II (AY229462, AY229346, AY229499, and AY229404), Lineage III (KJ504139, HQ686043, KJ504116, and DQ988349), and Lineage IV (EU840690, EF030606). The result shows that the prevalence of in Surabaya contaminating the chicken meat samples from the supermarkets was 10% (2/20), from the mobile vendors was 0/20 (0%), and from the traditional markets was 5% (1/20). It was seen from the band at 456 bp fragment. Furthermore, three isolates found in Surabaya were included in the new lineages which were resistant to old-generation antibiotics such as sulfamethonazole-trimetophrim (SXT) and amoxyllin sulbactam (MAS), but they were still sensitive to new-generation antibiotics such as cefotaxime (CTX) and meropenem (MEM).
本研究的目的是确定泗水市受污染鸡肉的系统发育分析和抗生素耐药性。从泗水市的超市、流动摊贩和传统市场收集了60份鸡肉样本。通过切碎25克鸡肉并将其放入灭菌的锥形瓶中，使用选择性培养基进行分离和鉴定。采用了一些方法进行鉴定程序，如生化和形态学测试、抗生素耐药性测试、PCR和测序；还使用Genetix Mac ver 8.0通过邻接法进行系统发育分析，参考GenBank中记录的hlyA基因，如谱系I（KC808543）、谱系II（AY229462、AY229346、AY229499和AY229404）、谱系III（KJ504139、HQ686043、KJ504116和DQ988349）以及谱系IV（EU840690、EF030606）。结果表明，泗水市超市鸡肉样本中该菌的污染率为10%（2/20），流动摊贩鸡肉样本中为0/20（0%），传统市场鸡肉样本中为5%（1/20）。在456 bp片段处可见条带。此外，在泗水市发现的三株分离株属于新谱系，它们对磺胺甲恶唑-甲氧苄啶（SXT）和阿莫西林舒巴坦（MAS）等第一代抗生素耐药，但对头孢噻肟（CTX）和美罗培南（MEM）等新一代抗生素仍敏感。
