Department of Biomaterial Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
Student Research Committee, Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Neural Regen Res. 2013 Aug 25;8(24):2266-74. doi: 10.3969/j.issn.1673-5374.2013.24.006.
It has been confirmed that nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nerve conduit can promote peripheral nerve regeneration in rats. However, its efficiency in repair of over 30-mm-long sciatic nerve defects needs to be assessed. In this study, we used a nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nerve conduit to bridge a 30-mm-long gap in the rat sciatic nerve. At 4 months after nerve conduit implantation, regenerated nerves were cally observed and histologically assessed. In the nanofibrous graft, the rat sciatic nerve trunk had been reconstructed by restoration of nerve continuity and formation of myelinated nerve fiber. There were Schwann cells and glial cells in the regenerated nerves. Masson's trichrome staining showed that there were no pathological changes in the size and structure of gastrocnemius muscle cells on the operated side of rats. These findings suggest that nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nerve conduit is suitable for repair of long-segment sciatic nerve defects.
已证实纳米纤维聚（3-羟基丁酸酯-共-3-羟基戊酸酯）神经导管能促进大鼠周围神经再生。然而，其修复 30mm 以上长坐骨神经缺损的效率仍需评估。本研究采用纳米纤维聚（3-羟基丁酸酯-共-3-羟基戊酸酯）神经导管桥接大鼠坐骨神经 30mm 缺损。神经导管植入 4 个月后，对再生神经进行大体观察和组织学评估。在纳米纤维移植物中，通过恢复神经连续性和形成有髓神经纤维，大鼠坐骨神经干得到重建。再生神经中有雪旺细胞和神经胶质细胞。Masson 三色染色显示，大鼠手术侧腓肠肌细胞的大小和结构无病理性改变。这些发现表明，纳米纤维聚（3-羟基丁酸酯-共-3-羟基戊酸酯）神经导管适用于修复长节段坐骨神经缺损。
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