半自动化商业重复序列 PCR 方法与 spoligotyping、24 位位基因间隔重复单位可变数目串联重复分型、IS6110 基于限制性片段长度多态性分析用于结核分枝杆菌分型的比较。

Comparison of a semiautomated commercial repetitive-sequence-based PCR method with spoligotyping, 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing, and restriction fragment length polymorphism-based analysis of IS6110 for Mycobacterium tuberculosis typing.

机构信息

AP-HP, Hôpital Pitié-Salpêtrière, Centre National de Référence des Mycobactéries et de la Résistance des Mycobactéries aux Antituberculeux, Paris, France Sorbonne Universités, UPMC Univ Paris 06, CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI-Paris, Team 13 (Bacteriology), Paris, France INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI-Paris, Team 13 (Bacteriology), Paris, France

Institut de Génétique et Microbiologie, UMR8621 CNRS-Université Paris-Sud 11, UniverSud, Infection Genetics Emerging Pathogens Evolution (IGEPE) Team, Orsay-Cedex, France.

出版信息

J Clin Microbiol. 2014 Nov;52(11):4082-6. doi: 10.1128/JCM.02226-14. Epub 2014 Sep 10.

DOI:10.1128/JCM.02226-14

PMID:25210067

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4313259/

Abstract

Fifty-two multidrug-resistant isolates of Mycobacterium tuberculosis representative of the currently predominant lineages in France were analyzed using repetitive-sequence-based PCR (rep-PCR) DiversiLab (DL), spoligotyping, 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing (MIRU-VNTR), and restriction fragment length polymorphism of IS6110 (IS6110-RFLP). DL, as opposed to MIRU-VNTR and IS6110-RFLP analysis, did not allow discrimination among half of the isolates, an indication of comparatively lower resolving power.

摘要

对代表法国目前主要谱系的 52 株耐多药结核分枝杆菌分离株进行了基于重复序列的 PCR（rep-PCR）DiversiLab（DL）、 spoligotyping、24 位位基因间隔重复单位可变数串联重复分型（MIRU-VNTR）和 IS6110 限制片段长度多态性（IS6110-RFLP）分析。与 MIRU-VNTR 和 IS6110-RFLP 分析不同，DL 分析不能区分一半的分离株，这表明其分辨率相对较低。