Devi M, Saraswathi T R, Ranganathan K, Vijayalakshmi D, Sreeja C, Fathima S Shabana
Department of Oral and Maxillofacial Pathology, Adiparasakthi Dental College, Melmaruvathur, Chennai, Tamil Nadu, India.
Department of Oral and Maxillofacial Pathology, Ragas Dental College, Uthandi, Tamil Nadu, India.
J Pharm Bioallied Sci. 2014 Jul;6(Suppl 1):S146-9. doi: 10.4103/0975-7406.137427.
The aim of this study is to identify and evaluate Langerhans cell (LC) in lichen planus (LP), lichenoid mucositis (LM) and normal mucosa (NM) using CD1a monoclonal antibody immunohistochemically.
A total of 15 cases of oral lichen planus and 15 cases of LM were selected based on clinical examination and confirmed by histopathological analysis. The biopsies from the 10 patients were taken from normal buccal mucosa as control. Paraffin blocks of tissue were made, which are used for routine hematoxylin and eosin staining and immunohistochemical staining using biotin streptavidin methods (CD1a monoclonal antibody). Analysis of CD1a expression was performed by evaluating the labeling index (LI) for each slide.
The mean CD1a LI for LP was significantly higher than that of LM and NM in the basal and supra basal layer. The mean CD1a positive cells in the connective tissues for LP were higher than that of LM and NM.
This study clearly demonstrates a statistically significant increase in number of LC in LP than in LM, indicating the possible different immunopathogenic mechanisms.
本研究旨在采用CD1a单克隆抗体免疫组织化学方法，鉴定和评估扁平苔藓（LP）、苔藓样黏膜炎（LM）及正常黏膜（NM）中的朗格汉斯细胞（LC）。
基于临床检查选取15例口腔扁平苔藓患者和15例苔藓样黏膜炎患者，并经组织病理学分析确诊。取10例患者的活检组织作为对照，取自正常颊黏膜。制作组织石蜡块，用于常规苏木精和伊红染色以及采用生物素-链霉亲和素法（CD1a单克隆抗体）进行免疫组织化学染色。通过评估每张切片的标记指数（LI）对CD1a表达进行分析。
LP的基底及基底上层平均CD1a LI显著高于LM和NM。LP结缔组织中的平均CD1a阳性细胞高于LM和NM。
本研究清楚地表明，LP中LC的数量在统计学上显著多于LM，提示可能存在不同的免疫致病机制。
