Farooq Shukkur M, Kumar Ashok, Ashour Hossam M
Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA.
J Cell Mol Med. 2014 Dec;18(12):2512-8. doi: 10.1111/jcmm.12376. Epub 2014 Sep 11.
Type II collagen (CII) is a cartilage structural protein that plays important roles in joint function, arthritis and ageing. In studying the ability of CII to induce eye-mediated specific immune tolerance, we have recently proven that CII is capable of inducing anterior chamber-associated immune deviation (ACAID) in Balb/c mice. Here, we study the ability of CII to induce eye-mediated immune tolerance in strains of mice that are prone to the induction of rheumatoid arthritis. Thus, we hypothesized that CII induces ACAID in DBA/1 mice and in C57BL/6 mice through the AC route (direct injection) or the intravenous route (adoptive transfer of in vitro-generated CII-specific ACAID macrophages or of CII-specific in vitro-generated T regulatory cells). Specific immune tolerance induction was assessed using both delayed-type hypersensitivity (DTH) and local adoptive transfer (LAT) assays. Results indicated the ability of CII to generate CII-specific ACAID-mediated immune tolerance in vivo and in vitro in both DBA/1 mice and C57BL/6 mice. These findings could be beneficial in studies of immune tolerance induction using CII.
II型胶原蛋白（CII）是一种软骨结构蛋白，在关节功能、关节炎和衰老过程中发挥着重要作用。在研究CII诱导眼介导的特异性免疫耐受的能力时，我们最近证实CII能够在Balb/c小鼠中诱导前房相关免疫偏离（ACAID）。在此，我们研究CII在易诱发类风湿性关节炎的小鼠品系中诱导眼介导免疫耐受的能力。因此，我们假设CII通过前房途径（直接注射）或静脉途径（体外产生的CII特异性ACAID巨噬细胞或体外产生的CII特异性调节性T细胞的过继转移）在DBA/1小鼠和C57BL/6小鼠中诱导ACAID。使用迟发型超敏反应（DTH）和局部过继转移（LAT）试验评估特异性免疫耐受的诱导情况。结果表明，CII能够在DBA/1小鼠和C57BL/6小鼠体内和体外产生CII特异性ACAID介导的免疫耐受。这些发现可能有助于使用CII进行免疫耐受诱导的研究。
