Osaka Yuhigaoka Gakuen Junior College, 7-72 Ikutamadera-cho, Tennoji, Osaka 543-0073, Japan; Division of Applied Life Sciences, Graduate School of Life and Environment Sciences, Kyoto Prefectural University, 1-5 Shimogamo, Kyoto 606-8522, Japan.
Division of Applied Life Sciences, Graduate School of Life and Environment Sciences, Kyoto Prefectural University, 1-5 Shimogamo, Kyoto 606-8522, Japan.
Food Chem. 2011 Dec 1;129(3):1019-24. doi: 10.1016/j.foodchem.2011.05.066. Epub 2011 May 23.
Peptides in the blood of subjects before and after collagen hydrolysate ingestion were fractionated by ion exchange and size-exclusion chromatographies and then derivatised with phenyl isothiocyanate. The derivatives were characterised by reserved phase (RP)-HPLC. Prolyl-hydroxyproline (Pro-Hyp), which has been identified in the previous studies, was detected as a major food-derived collagen peptide in the blood of all subjects (n=5). Another major peptide was identified as hydroxyprolyl-glycine (Hyp-Gly) in the blood of four subjects, which has not been detected in previous studies. The ratio of Hyp-Gly to Pro-Hyp depended on subjects and ranged from 0.00 to 5.04. Hyp-Gly was less susceptible to human serum peptidase than Pro-Hyp. Hyp-Gly enhanced the growth of mouse primary fibroblasts on collagen gels in a higher extent than Pro-Hyp. These findings suggest that Hyp-Gly plays a significant role in exerting the biological effects by ingestion of collagen hydrolysate.
受试者在摄入胶原蛋白水解物前后的血液中的肽段分别通过离子交换和排阻色谱进行分离，然后用苯异硫氰酸进行衍生化。所得衍生物通过保留相（RP）-HPLC 进行表征。脯氨酰-羟脯氨酸（Pro-Hyp）在前一项研究中已被鉴定为主要的食物来源的胶原蛋白肽，在所有受试者（n=5）的血液中均有检测到。另一种主要的肽在四名受试者的血液中被鉴定为羟脯氨酸-甘氨酸（Hyp-Gly），在之前的研究中没有检测到。Hyp-Gly 与 Pro-Hyp 的比值取决于受试者，范围从 0.00 到 5.04。Hyp-Gly 比 Pro-Hyp 更不易受人体血清肽酶的影响。Hyp-Gly 比 Pro-Hyp 更能促进小鼠原代成纤维细胞在胶原蛋白凝胶上的生长。这些发现表明，Hyp-Gly 在摄入胶原蛋白水解物后发挥生物效应中起着重要作用。
