Taga Yuki, Iwasaki Yu, Tometsuka Chisa, Funato Noriko, Shigemura Yasutaka, Kusubata Masashi, Mizuno Kazunori
Nippi Research Institute of Biomatrix, 520-11 Kuwabara, Toride, Ibaraki, 302-0017, Japan.
Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi-ku, Tokyo, 173-8602, Japan.
NPJ Sci Food. 2022 Jun 3;6(1):29. doi: 10.1038/s41538-022-00144-4.
There are increasing reports demonstrating high bioavailability of 4-hydroxyproline (4Hyp)-containing oligopeptides after oral ingestion of collagen hydrolysate and their bioactivity. In contrast, no study investigates the fate of another collagen-specific but minor amino acid, 3Hyp. Here, we identified Gly-3Hyp-4Hyp tripeptide in human blood at high concentrations, comparable to other 4Hyp-containing oligopeptides, after ingesting porcine skin collagen hydrolysate. Additionally, Gly-3Hyp-4Hyp uniquely maintained the maximum concentration until 4 h after the ingestion due to its exceptionally high resistance to peptidase/protease demonstrated by incubation with mouse plasma. In mice, oral administration of collagen hydrolysate prepared from bovine tendon, which contains a higher amount of 3Hyp, further increased blood Gly-3Hyp-4Hyp levels compared to that from bovine skin. Furthermore, Gly-3Hyp-4Hyp showed chemotactic activity on skin fibroblasts and promoted osteoblast differentiation. These results highlight the specific nature of the Gly-3Hyp-4Hyp tripeptide and its potential for health promotion and disease treatment.
越来越多的报告表明，口服胶原蛋白水解物后，含4-羟基脯氨酸（4Hyp）的寡肽具有很高的生物利用度及其生物活性。相比之下，尚无研究调查另一种胶原蛋白特有的但含量较少的氨基酸——3-羟基脯氨酸（3Hyp）的去向。在此，我们发现，在摄入猪皮胶原蛋白水解物后，人体血液中甘氨酰-3Hyp-4Hyp三肽的浓度很高，与其他含4Hyp的寡肽相当。此外，由于与小鼠血浆孵育显示甘氨酰-3Hyp-4Hyp对肽酶/蛋白酶具有极高的抗性，其独特地在摄入后4小时内维持最大浓度。在小鼠中，口服由牛肌腱制备的胶原蛋白水解物（其中3Hyp含量较高）后，与牛皮肤来源的相比，血液中甘氨酰-3Hyp-4Hyp水平进一步升高。此外，甘氨酰-3Hyp-4Hyp对皮肤成纤维细胞具有趋化活性，并促进成骨细胞分化。这些结果突出了甘氨酰-3Hyp-4Hyp三肽的特性及其在促进健康和疾病治疗方面的潜力。
