Fernandez-Botran R, Sanders V M, Vitetta E S
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
J Exp Med. 1989 Feb 1;169(2):379-91. doi: 10.1084/jem.169.2.379.
IL-2 and IL-4 induce a synergistic proliferative response in HT-2 cells, suggesting that IL-2Rs and IL-4Rs may interact. The purpose of this study was to examine the effect of IL-4 on the expression and function of IL-2Rs. Preincubation of HT-2 and BCL1-3B3 cells with IL-4 for 60 min at 4 degrees C or 37 degrees C resulted in a partial decrease in the number, but not the affinity of high affinity IL-2Rs as evidenced by Scatchard analysis of binding data. The decrease in the number of high affinity receptors correlated with decreased internalization of IL-2. After preincubation with IL-4, crosslinking of 125I-IL-2 to high affinity IL-2Rs also demonstrated a approximately 50% reduction in the number of high affinity IL-2Rs. Another lymphokine, IL-1, which acts on HT-2 cells, had no measurable effect on the affinity or number of IL-2Rs. Taken together, these results indicate that IL-4 downregulates the expression of high affinity IL-2Rs on some cells. It is not known whether this occurs by a direct ligand-mediated receptor interaction, by the sharing of a common receptor subunit, or by interaction of the two receptors with another membrane molecule or cytoskeletal component.
白细胞介素-2（IL-2）和白细胞介素-4（IL-4）可在HT-2细胞中诱导协同增殖反应，这表明IL-2受体（IL-2Rs）和IL-4受体（IL-4Rs）可能相互作用。本研究的目的是检测IL-4对IL-2Rs表达和功能的影响。在4℃或37℃下，将HT-2和BCL1-3B3细胞与IL-4预孵育60分钟，结果显示高亲和力IL-2Rs的数量部分减少，但亲和力未变，结合数据的Scatchard分析证明了这一点。高亲和力受体数量的减少与IL-2内化减少相关。用IL-4预孵育后，125I-IL-2与高亲和力IL-2Rs的交联也显示高亲和力IL-2Rs的数量减少了约50%。另一种作用于HT-2细胞的淋巴因子IL-1，对IL-2Rs的亲和力或数量没有可测量的影响。综上所述，这些结果表明IL-4可下调某些细胞上高亲和力IL-2Rs的表达。尚不清楚这是通过直接的配体介导的受体相互作用、共享共同的受体亚基，还是通过两种受体与另一种膜分子或细胞骨架成分的相互作用而发生的。
