Michopoulou Vasiliki, Derdas Stavros P, Symvoulakis Emmanouil, Mourmouras Nikolaos, Nomikos Alexandros, Delakas Dimitris, Sourvinos George, Spandidos Demetrios A
Laboratory of Virology, Faculty of Medicine, University of Crete, Heraklion, 71003, Crete, Greece.
Tumour Biol. 2014 Dec;35(12):12765-73. doi: 10.1007/s13277-014-2604-7. Epub 2014 Sep 12.
Prostate cancer is the most common neoplasm found in males and the second most frequent cause of cancer-related mortality in males in Greece. Among other pathogens, the detection frequency of human papillomavirus (HPV) has been found to be significantly increased in tumor tissues among patients with sexually transmitted diseases (STDs), depending on the geographical distribution of each population studied. The present study focused on the detection of HPV and the distribution of Arg72Pro p53 polymorphism in a cohort of healthy individuals, as well as prostate cancer patients. We investigated the presence of HPV in 50 paraffin-embedded prostate cancer tissues, as well as in 30 physiological tissue samples from healthy individuals by real-time PCR. Furthermore, the same group of patients was also screened for the presence of the Arg72Pro polymorphism of the p53 gene, a p53 polymorphism related to HPV. Out of the 30 control samples, only 1 was found positive for HPV (3.33 %). On the contrary, HPV DNA was detected in 8 out of the total 50 samples (16 %) in the prostate cancer samples. The distribution of the three genotypes, Arg/Arg, Arg/Pro, and Pro/Pro, was 69.6, 21.7, and 8.7 % in the cancer patients and 75.0, 17.86, and 7.14 % in healthy controls, respectively. No statistically significant association was observed between the HPV presence and the age, stage, p53 polymorphism status at codon 72, or PSA. The increased prevalence of HPV detected in the prostate cancer tissues is in agreement with that reported in previous studies, further supporting the association of HPV infection and prostate cancer.
前列腺癌是男性中最常见的肿瘤，也是希腊男性癌症相关死亡的第二大常见原因。在其他病原体中，已发现性传播疾病（STD）患者的肿瘤组织中人类乳头瘤病毒（HPV）的检测频率显著增加，这取决于所研究的每个群体的地理分布。本研究聚焦于健康个体队列以及前列腺癌患者中HPV的检测和Arg72Pro p53多态性的分布。我们通过实时聚合酶链反应（PCR）研究了50个石蜡包埋的前列腺癌组织以及30个来自健康个体的生理组织样本中HPV的存在情况。此外，还对同一组患者进行了p53基因Arg72Pro多态性（一种与HPV相关的p53多态性）的筛查。在30个对照样本中，仅1个被发现HPV呈阳性（3.33%）。相反，在50个前列腺癌样本中，共8个检测到HPV DNA（16%）。三种基因型Arg/Arg、Arg/Pro和Pro/Pro在癌症患者中的分布分别为69.6%、21.7%和8.7%，在健康对照中分别为75.0%、17.86%和7.14%。未观察到HPV的存在与年龄、分期、密码子72处的p53多态性状态或前列腺特异性抗原（PSA）之间存在统计学上的显著关联。在前列腺癌组织中检测到的HPV患病率增加与先前研究报告一致，进一步支持了HPV感染与前列腺癌之间的关联。
