Leiros Gustavo J, Galliano Silvia R, Sember Mario E, Kahn Tomas, Schwarz Elisabeth, Eiguchi Kumiko
Facultad de Medicina-Universidad del Salvador, Buenos Aires, Argentina.
BMC Urol. 2005 Nov 24;5:15. doi: 10.1186/1471-2490-5-15.
Infections with high-risk human papillomaviruses (HPVs), causatively linked to cervical cancer, might also play a role in the development of prostate cancer. Furthermore, the polymorphism at codon 72 (encoding either arginine or proline) of the p53 tumor-suppressor gene is discussed as a possible determinant for cancer risk. The HPV E6 oncoprotein induces degradation of the p53 protein. The aim of this study was to analyse prostate carcinomas and hyperplasias of patients from Argentina for the presence of HPV DNA and the p53 codon 72 polymorphism genotype.
HPV DNA detection and typing were done by consensus L1 and type-specific PCR assays, respectively, and Southern blot hybridizations. Genotyping of p53 codon 72 polymorphism was performed both by allele specific primer PCRs and PCR-RFLP (Bsh1236I). Fischer's test with Woolf's approximation was used for statistical analysis.
HPV DNA was detected in 17 out of 41 (41.5%) carcinoma samples, whereas all 30 hyperplasia samples were HPV-negative. Differences in p53 codon 72 allelic frequencies were not observed, neither between carcinomas and hyperplasias nor between HPV-positive and HPV-negative carcinomas.
These results indicate that the p53 genotype is probably not a risk factor for prostate cancer, and that HPV infections could be associated with at least a subset of prostate carcinomas.
高危型人乳头瘤病毒（HPV）感染与宫颈癌存在因果关系，可能也在前列腺癌的发生中起作用。此外，p53肿瘤抑制基因第72位密码子（编码精氨酸或脯氨酸）的多态性被认为可能是癌症风险的一个决定因素。HPV E6癌蛋白可诱导p53蛋白降解。本研究旨在分析阿根廷患者的前列腺癌和前列腺增生组织中HPV DNA的存在情况以及p53第72位密码子多态性基因型。
分别采用L1共识PCR和型特异性PCR检测HPV DNA并进行分型，同时进行Southern杂交。采用等位基因特异性引物PCR和PCR-RFLP（Bsh1236I）对p53第72位密码子多态性进行基因分型。采用Fischer检验及Woolf近似法进行统计学分析。
41例癌组织样本中有17例（41.5%）检测到HPV DNA，而30例增生组织样本均为HPV阴性。未观察到p53第72位密码子等位基因频率在癌组织与增生组织之间以及HPV阳性与阴性癌组织之间存在差异。
这些结果表明，p53基因型可能不是前列腺癌的风险因素，且HPV感染可能与至少一部分前列腺癌相关。
