Semenoff Tereza Aparecida Delle Vedove, Vieira Evanice Menezes Marçal, Borges Alvaro Henrique, Bandeca Matheus Coelho, Dos Santos Reidson Stanley Soares, Pedro Fábio Luis Miranda, da Silva Natalino Francisco, Semenoff-Segundo Alex
Professor, Department of Master Program in Integrated Dentistry Science, University of Cuiabá-UNIC, Cuiabá, MT, Brazil.
Professor, Department of Master Program in Dentistry, CEUMA University-UniCEUMA, São Luis, Brazil.
J Int Oral Health. 2014 Jul;6(4):4-8.
The Calcium Hydroxide has been widely used as an intracanal dressing and in combination with restorative and endodontic materials and its main goal is the tissue reparation. However, when the patient has chronic stress, the immunological response and tissue repair decreases in both the epithelial and connective tissue. Therefore, the aim was to analyze the effect of chronic stress on the tissue response in rats exposed to calcium hydroxide (CH).
A total of 60 wistar rats were anesthetized, and a polyethylene tube containing CH was inserted under the skin. After 24 h, they were divided into two groups: Calcium hydroxide + stress (CHSG) n = 30 and calcium hydroxide (CHG) n = 30. They were stressed by physical restraint, for 12 h each day for periods of 7, 15 and 30 days when 10 animals from each group were euthanized. The tissues surrounding the polyethylene tubes were removed, and slides were prepared and stained with hematoxylin and eosin. The analysis was performed with an optical microscope with magnification of 4-400 times by a blinded senior examiner. The sample slides were classified according to the following scores 0 - absent/1 - present/2 - infiltrate to: Inflammatory infiltrate containing fibrous condensation, lymphocytes, plasmacytes, macrophages, neutrophils, and eosinophils. The data were statistically analyzed using the Student's t-test (P < 0.05) for paired samples.
The exposure time of 7 days elicited no statistical difference between groups (P > 0.05). The 15 days exposure group had higher averages for CHG to eosinophils and inflammatory infiltrate (P < 0.05). In 30 days, CHG showed higher averages to inflammatory infiltrate and lower averages to FC (P < 0.05).
Some modified patterns of responses in the CHSG were observed at 15 days and 30 days.
氢氧化钙已被广泛用作根管内敷料，并与修复和牙髓材料联合使用，其主要目的是组织修复。然而，当患者处于慢性应激状态时，上皮组织和结缔组织的免疫反应及组织修复都会降低。因此，本研究旨在分析慢性应激对暴露于氢氧化钙（CH）的大鼠组织反应的影响。
总共60只Wistar大鼠麻醉后，将含有CH的聚乙烯管植入皮下。24小时后，将它们分为两组：氢氧化钙+应激组（CHSG），n = 30；氢氧化钙组（CHG），n = （此处原文有误，应为30）。通过物理束缚使其产生应激，每天12小时，持续7天、15天和30天，每组分别有10只动物在相应时间点安乐死。取出聚乙烯管周围的组织，制备玻片并用苏木精和伊红染色。由一位不知情的资深检查人员使用放大倍数为4 - 400倍的光学显微镜进行分析。样本玻片根据以下评分分类：0 - 无/1 - 有/2 - 浸润，包括含有纤维性凝聚、淋巴细胞、浆细胞、巨噬细胞、中性粒细胞和嗜酸性粒细胞的炎性浸润。使用配对样本的Student's t检验（P < 0.05）对数据进行统计学分析。
7天暴露时间组间无统计学差异（P > 0.05）。15天暴露组中，CHG组嗜酸性粒细胞和炎性浸润的平均值较高（P < 0.05）。30天时，CHG组炎性浸润平均值较高，纤维性凝聚平均值较低（P < 0.05）。
在15天和30天时观察到CHSG组有一些反应模式的改变。
