大鼠结缔组织对一种新型氢氧化钙基封闭剂的反应。

Reaction of rat connective tissue to a new calcium hydroxide-based sealer.

作者信息

Gomes-Filho João Eduardo, Bernabé Pedro Felício Estrada, Nery Mauro Juvenal, Otoboni-Filho José Arlindo, Dezan-Júnior Eloi, de Moraes Costa Mariana Machado Teixeira, de Faria Max Douglas, Watanabe Simone, Gomes Alessandra Cristina

机构信息

Department of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, Brazil.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Aug;106(2):e71-6. doi: 10.1016/j.tripleo.2008.03.030. Epub 2008 Jun 13.

DOI:10.1016/j.tripleo.2008.03.030

PMID:18554956

Abstract

The aim of this study was to histopathologically examine the reaction of the connective tissue of rats to 2 calcium hydroxide-based sealers, Acroseal and Sealapex. Dentin tubes containing the materials and empty control tubes were implanted into the dorsal connective tissue of 36 Wistar albino rats. The animals were killed after 7 or 30 days, and the specimens were prepared for histologic analysis with hematoxylin and eosin, Von Kossa technique, and polarized light. Results were statistically analyzed using Kruskal-Wallis test. Both materials caused mild or moderate inflammatory reactions on the 7th day, but these reactions decreased by the 30th day with no significant difference at any time (P > .05). Mineralization of the subcutaneous tissue of the rats was observed only with Sealapex.

摘要

本研究的目的是对大鼠结缔组织对两种氢氧化钙类封闭剂Acroseal和Sealapex的反应进行组织病理学检查。将含有材料的牙本质小管和空的对照小管植入36只Wistar白化大鼠的背部结缔组织中。在7天或30天后处死动物，并使用苏木精和伊红、冯·科萨技术以及偏振光对标本进行组织学分析。使用Kruskal-Wallis检验对结果进行统计学分析。两种材料在第7天均引起轻度或中度炎症反应，但到第30天这些反应有所减轻，且在任何时间均无显著差异（P>.05）。仅在使用Sealapex时观察到大鼠皮下组织的矿化。