Eagle Mark J, Rooney Paul, Kearney John N
NHSBT, Tissue Services R&D, 14 Estuary Banks, Speke, Liverpool, L24 8RB, UK.
J Biomed Mater Res B Appl Biomater. 2015 Jul;103(5):1023-9. doi: 10.1002/jbm.b.33280. Epub 2014 Sep 15.
Human demineralized bone matrix derived from cortical bone is used by surgeons due to its ability to promote bone formation. There is also a need for shaped demineralized bone matrices made from cancellous bone, where the properties of the material allow its insertion into defects, therefore acting as a void filler and scaffold onto which new bone can form. In this study, we report that demineralized bone sponges were prepared by dissecting and cutting knee bone into cancellous bone cubes of 1 cm(3) . These cubes were then taken through a series of warm water washes, some with sonication, centrifugation, and two decontamination chemical washes. The cubes were optimally demineralized into sponges with 0.5N hydrochloric acid under vacuum with constant pH measurement. Demineralization was confirmed by quantitative measurement of calcium and qualitatively by compression. The sponges were freeze dried before terminal sterilisation with a target dose of 25 kGy gamma radiation whilst frozen. Samples of the sponges were histologically examined for calcium and collagen and also tested for osteoinductivity. Data showed well defined collagen staining in the sponges, with little residual calcium. Sponges from two out of three donors demonstrated osteoinductivity when implanted into the muscle of an athymic mouse.
外科医生使用源自皮质骨的人脱矿骨基质，因为它具有促进骨形成的能力。也需要由松质骨制成的成型脱矿骨基质，这种材料的特性使其能够插入缺损处，因此可作为空隙填充物和新骨能够在其上形成的支架。在本研究中，我们报告通过将膝关节骨解剖并切割成1立方厘米的松质骨立方体来制备脱矿骨海绵。然后将这些立方体进行一系列温水冲洗，有些冲洗还伴有超声处理、离心以及两次去污化学冲洗。在持续测量pH值的真空条件下，用0.5N盐酸将立方体最佳地脱矿化为海绵。通过钙的定量测量和压缩的定性分析来确认脱矿。在最终灭菌前，将海绵冷冻干燥，目标剂量为25千戈瑞的伽马辐射，同时保持冷冻状态。对海绵样本进行组织学检查以检测钙和胶原蛋白，并测试其骨诱导活性。数据显示海绵中有明确的胶原蛋白染色，几乎没有残留钙。当植入无胸腺小鼠的肌肉中时，来自三个供体中的两个供体的海绵表现出骨诱导活性。
