Iadarola M J, Mouradian M M
Neurobiology and Anesthesiology Branch, National Institute of Dental Research, Bethesda, MD 20892.
Brain Res. 1989 Feb 13;479(2):397-401. doi: 10.1016/0006-8993(89)91648-x.
The cerebrospinal fluid (CSF) content of Met5-enkephalin-Arg-Gly-Leu immunoreactivity was found to be significantly decreased in patients with Huntington's disease and progressive supranuclear palsy. This peptide is derived from the proenkephalin precursor protein and normally is found in high concentrations in the basal ganglia. The decrease in CSF from Huntington's disease patients likely reflects the loss of proenkephalin-containing neurons seen in postmortem analyses of basal ganglia tissue. The decrease in progressive supranuclear palsy, a disease in which dopamine neurons degenerate but enkephalin levels in the basal ganglia are reportedly not decreased, may reflect a functional decline in enkephalinergic neuronal activity secondary to a striatal cholinergic deficit. The results suggest that a substantial portion of the CSF Met5-enkephalin-Arg-Gly-Leu immunoreactivity is derived from the basal ganglia and that CSF levels of this peptide can serve as an index of functional or anatomical integrity of proenkephalin synthesizing neurons in the basal ganglia.
在亨廷顿舞蹈症和进行性核上性麻痹患者中，发现脑脊液（CSF）中Met5-脑啡肽-精氨酸-甘氨酸-亮氨酸免疫反应性显著降低。这种肽来源于前脑啡肽原蛋白，正常情况下在基底神经节中含量很高。亨廷顿舞蹈症患者脑脊液中该物质的减少可能反映了在基底神经节组织的尸检分析中所见到的含前脑啡肽神经元的丧失。在进行性核上性麻痹中，多巴胺神经元退化，但据报道基底神经节中的脑啡肽水平并未降低，其脑脊液中该物质的减少可能反映了纹状体胆碱能缺陷继发的脑啡肽能神经元活动的功能衰退。结果表明，脑脊液中大部分Met5-脑啡肽-精氨酸-甘氨酸-亮氨酸免疫反应性来源于基底神经节，并且该肽的脑脊液水平可作为基底神经节中前脑啡肽合成神经元功能或解剖完整性的一个指标。
