人乳头瘤病毒感染及Stathmin在鼻窦内翻性乳头状瘤发病机制中的作用

Roles of human papillomavirus infection and stathmin in the pathogenesis of sinonasal inverted papilloma.

作者信息

Lin Hai, Lin Dong, Xiong Xi-Sheng

机构信息

Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, China.

Department of Otorhinolaryngology, Fuzhou General Hospital, Fuzhou, Fujian, China.

出版信息

Head Neck. 2016 Feb;38(2):220-4. doi: 10.1002/hed.23864. Epub 2015 May 22.

DOI:10.1002/hed.23864

PMID:25224680

Abstract

BACKGROUND

The purpose of this study was to investigate roles of human papillomavirus (HPV) infection and stathmin in sinonasal inverted papilloma (SNIP).

METHODS

HPV DNA detection was performed by the fluorescence-based polymerase chain reaction (PCR) method. Stathmin protein expression was investigated by the immunohistochemistry method and mRNA expression of stathmin, Kif2a, and cyclin D1 were assessed by real-time PCR in SNIP and control subjects.

RESULTS

The positive rate of HPV DNA detected in SNIP was about 53.6% (15 of 28). Recurrent cases showed a higher rate of HPV infection compared with initial cases and higher Krouse stage (T3 + T4) cases showed higher rate of HPV infection than lower Krouse stage (T1 + T2) cases. Stronger expression of stathmin, Kif2a, and cyclin D1 were observed in SNIP, especially HPV(+) SNIP.

CONCLUSION

HPV infection was closely associated with recurrence and progression of SNIP. Stathmin is a valuable prognostic marker and could be considered as a therapeutic target in patients with SNIP.

摘要

背景

本研究旨在探讨人乳头瘤病毒（HPV）感染和微管相关蛋白在鼻窦内翻性乳头状瘤（SNIP）中的作用。

方法

采用基于荧光的聚合酶链反应（PCR）法检测HPV DNA。通过免疫组织化学方法研究微管相关蛋白的表达，并采用实时PCR评估微管相关蛋白、驱动蛋白家族成员2A（Kif2a）和细胞周期蛋白D1在SNIP和对照受试者中的mRNA表达。

结果

在SNIP中检测到的HPV DNA阳性率约为53.6%（28例中的15例）。与初发病例相比，复发病例的HPV感染率更高，与Krouse分期较低（T1+T2）的病例相比，Krouse分期较高（T3+T4）的病例HPV感染率更高。在SNIP中观察到微管相关蛋白、Kif2a和细胞周期蛋白D1的表达更强，尤其是HPV（+）的SNIP。