马来西亚甲型流感病毒基因型的鉴定。
Identification of genotypes of Influenza A virus in Malaysia.
机构信息
Rahman MM, Professor, Dept. of Medical Microbiology & Immunology, Faculty of Medicine, UKM, Cheras 56000, Kuala Lumpur, Malaysia.
Wong KK, Scholar, Dept. of Medical Microbiology & Immunology, Faculty of Medicine, UKM, Cheras 56000, Kuala Lumpur, Malaysia.
出版信息
Pak J Med Sci. 2014 Sep;30(5):1068-71. doi: 10.12669/pjms.305.5224.
OBJECTIVE
Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia.
METHODS
Influenza A virus was identified from respiratory specimens by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). Phylogenetic analysis of the identified isolates was performed and genotypes were detected.
RESULTS
A total number of 505 throat swabs and nasopharyngeal aspirates were examined by rRT-PCR at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) in which 65(12.87%) were positive for influenza A. The identified isolates were successfully genotyped by phylogenetic analysis. The identified influenza A genotypes were: H1N1 (42), H3N2 (20) and H5N1 (3).
CONCLUSION
The findings indicated that 3 genotypes were circulating in Malaysia during 2011 in which H1N1 was the predominant. RESULTS added new genotype (H5N1) identification record in Malaysia that may be added in data base of WHO and CDC.
目的
流感至今仍被认为是一种新发疾病。本研究旨在确定马来西亚甲型流感病毒的流行基因型。
方法
通过实时逆转录聚合酶链反应（rRT-PCR）从呼吸道标本中鉴定甲型流感病毒。对鉴定出的分离株进行系统发育分析，并检测基因型。
结果
在马来西亚国民大学医学中心（UKMMC），共检测了 505 份咽喉拭子和鼻咽抽吸物的 rRT-PCR，其中 65 份（12.87%）甲型流感病毒阳性。通过系统发育分析成功对鉴定出的分离株进行了基因分型。鉴定出的甲型流感基因型为：H1N1（42）、H3N2（20）和 H5N1（3）。
结论
研究结果表明，2011 年马来西亚有 3 种基因型流行，其中 H1N1 占优势。研究结果增加了在马来西亚新型 H5N1 基因型的鉴定记录，该记录可能被添加到世界卫生组织和疾病预防控制中心的数据库中。
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