Rahman M M, Wong K K, Alfizah H, Hussin S, Isahak I
M. M. Rahman, Department of Medical Microbiology & Immunology, Faculty of Medicine, The National University of Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.
K.K. Wong, Department of Medical Microbiology & Immunology, Faculty of Medicine, The National University of Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.
Pak J Med Sci. 2015 Jul-Aug;31(4):791-4. doi: 10.12669/pjms.314.7003.
To determine the efficacy of cell culture, immunoflourescence Assay (IFA) and real time polymerase chain reaction (rRT-PCR) in relation to diagnosis of influenza and Respiratory Syncytial Virus (RSV).
Total 2781 specimens of throat swabs and nasopharyngeal aspirates were obtained from patients suspected of respiratory viruses' infections from January 2009 to December 2011 at Universiti Kebangsaan Malaysia Medical Centre(UKMMC). The specimens were processed by cell culture and immunoflurescence assay (IFA) and (rRT-PCR).
Thirty three (1.19%) specimens were positive for influenza virus A and 42 (1.51%) were positive for RSV by cell culture and IFA. On the other hand, rRT-PCR was able to identify 189 of 505 (37.43%) specimens in which 65 were influenza A virus and 124 were RSV. Sensitivity of rRT-PCR was 100% for both influenza A virus and RSV and specificity was 88% and 77% for influenza A virus and RSV, respectively.
rRT-PCR diagnosed respiratory viruses in shorter time with a high level of sensitivity in comparison to conventional assays - cell culture and IFA. These advantages help in managing patients by saving cost and hospitalization stay.
确定细胞培养、免疫荧光测定法（IFA）和实时聚合酶链反应（rRT-PCR）在流感和呼吸道合胞病毒（RSV）诊断中的效能。
2009年1月至2011年12月期间，从马来西亚国民大学医学中心（UKMMC）疑似呼吸道病毒感染的患者中获取了总共2781份咽拭子和鼻咽抽吸物标本。这些标本通过细胞培养、免疫荧光测定法（IFA）和（rRT-PCR）进行处理。
通过细胞培养和IFA，33份（1.19%）标本甲型流感病毒呈阳性，42份（1.51%）标本RSV呈阳性。另一方面，rRT-PCR能够在505份标本中鉴定出189份（37.43%），其中65份为甲型流感病毒，124份为RSV。rRT-PCR对甲型流感病毒和RSV的敏感性均为100%，对甲型流感病毒和RSV的特异性分别为88%和77%。
与传统检测方法——细胞培养和IFA相比，rRT-PCR能在更短时间内诊断呼吸道病毒，且敏感性高。这些优势有助于通过节省成本和缩短住院时间来管理患者。
