Li Jiao-Kun, Song Yun-Peng, Xu Hui, Zhu Jian-Yu, Tang Lu-Lu
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, People's Republic of China ; School of Metallurgy and Environment, Central South University, Changsha 410083, People's Republic of China.
School of Life Sciences, Central South University, Changsha 410013, People's Republic of China.
Appl Plant Sci. 2015 Jan 30;3(2). doi: 10.3732/apps.1400098. eCollection 2015 Feb.
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Microsatellite primers were developed for the pseudometallophyte Commelina communis (Commelinaceae), an important pioneer plant for phytoremediation of copper-contaminated soil. Two wild populations collected from metalliferous and nonmetalliferous sites were used to assess the polymorphism at each locus. •
Based on the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method, a total of 34 pairs of simple sequence repeat (SSR) markers were designed. When 40 specimens from two populations were screened, 12 microsatellite loci were found to be highly polymorphic. The number of alleles per locus ranged from one to 11 and the observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.195 to 0.941, respectively. •
These markers will be useful for examining genetic diversity, population structure, and gene flow in populations of C. communis under different edaphic conditions and guiding sustainable management plans for phytoremediation.
研究前提:为鸭跖草(鸭跖草科)开发了微卫星引物,鸭跖草是铜污染土壤植物修复的重要先锋植物。从含金属和不含金属的地点采集了两个野生种群,用于评估每个位点的多态性。
方法与结果:基于AFLP序列含重复序列快速分离(FIASCO)方法,共设计了34对简单序列重复(SSR)标记。当对两个种群的40个样本进行筛选时,发现12个微卫星位点具有高度多态性。每个位点的等位基因数从1到11不等,每个位点观察到的杂合度和预期杂合度分别从0.000到1.000和从0.195到0.941不等。
结论:这些标记将有助于研究不同土壤条件下鸭跖草种群的遗传多样性、种群结构和基因流,并指导植物修复的可持续管理计划。