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环状指蛋白参与巴雷特食管向食管腺癌的进展：一项初步研究。

RING finger proteins are involved in the progression of barrett esophagus to esophageal adenocarcinoma: a preliminary study.

作者信息

Wang Xing Wei, Wei Wei, Wang Wei Qiang, Zhao Xiao Yan, Guo Hong, Fang Dian Chun

机构信息

Department of Gastroenterology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China ; Gastroenterology Research Institute, Southwest Hospital, Third Military Medical University, Chongqing, China.

Department of Trauma and Microsurgery, 324 Hospital of People's Liberation Army, Chongqing, China.

出版信息

Gut Liver. 2014 Sep;8(5):487-94. doi: 10.5009/gnl13133. Epub 2014 Feb 24.

DOI:10.5009/gnl13133
PMID:25228972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4164246/
Abstract

BACKGROUND/AIMS: To investigate the differential expression of RING finger (RNF) proteins in Barrett esophagus (BE) and esophageal adenocarcinoma (EAC).

METHODS

The differential expression of RNFs in normal esophagus (NE), BE, and EAC was screened using microarray assay. Real-time quantitative polymerase chain reaction (PCR), tissue micro-array assay, and Western blot analysis were independently performed to detect the mRNA and protein expression of screened RNFs.

RESULTS

The expression of nine RNFs in the BE or EAC was 2-fold higher than those in NE. Among these proteins, the RNF32 and RNF121 expression in BE was 20.3-fold and 16.4-fold higher, respectively, than that in NE, and the expression of RNF24, RNF130, RNF141, RNF139, RNF11, RNF14, and RNF159 was upregulated more than 2-fold compared with NE. The expression of nine RNFs was not only upregulated in the EAC but was also positively related to the RNF expression in BE. The PCR results also indicated increased expression of these RNFs in BE and EAC compared to NE. Furthermore, the mRNA expression of all RNFs, except for RNF141 in EAC, was dramatically higher than those in the BE. Similar results were also obtained from the Western blot analysis.

CONCLUSIONS

A total of nine RNFs play critical roles in the progression of BE to EAC.

摘要

背景/目的：研究巴雷特食管（BE）和食管腺癌（EAC）中环状结构域（RNF）蛋白的差异表达。

方法

使用微阵列分析筛选正常食管（NE）、BE和EAC中RNF的差异表达。独立进行实时定量聚合酶链反应（PCR）、组织微阵列分析和蛋白质印迹分析，以检测筛选出的RNF的mRNA和蛋白表达。

结果

BE或EAC中9种RNF的表达比NE中的高2倍。在这些蛋白中，BE中RNF32和RNF121的表达分别比NE中高20.3倍和16.4倍，与NE相比，RNF24、RNF130、RNF141、RNF139、RNF11、RNF14和RNF159的表达上调超过2倍。9种RNF的表达不仅在EAC中上调，而且与BE中的RNF表达呈正相关。PCR结果还表明，与NE相比，这些RNF在BE和EAC中的表达增加。此外，除EAC中的RNF141外，所有RNF的mRNA表达均显著高于BE中的表达。蛋白质印迹分析也得到了类似结果。

结论

共有9种RNF在BE向EAC的进展中起关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/d44aa5659821/gnl-08-487f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/af9f138a83ef/gnl-08-487f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/d36215c3769f/gnl-08-487f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/605ee9258f8b/gnl-08-487f3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/18e702c4d87b/gnl-08-487f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/b1f84fe11479/gnl-08-487f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/d44aa5659821/gnl-08-487f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/af9f138a83ef/gnl-08-487f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/d36215c3769f/gnl-08-487f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/605ee9258f8b/gnl-08-487f3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/18e702c4d87b/gnl-08-487f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/b1f84fe11479/gnl-08-487f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/4164246/d44aa5659821/gnl-08-487f6.jpg

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