Ehrhardt Carolin, Arapitsas Panagiotis, Stefanini Marco, Flick Gerhard, Mattivi Fulvio
Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010, San Michele all´Adige, Italy; Department of Agriculture and Food Science, University of Applied Sciences Neubrandenburg, Brodaer Str. 2, 17033, Neubrandenburg, Germany.
J Mass Spectrom. 2014 Sep;49(9):860-9. doi: 10.1002/jms.3440.
The phenolic composition of grape berries grown in Italy (Cabernet Cortis, Johanniter, Solaris, Phoenix, and Regent) and in Germany (Regent and Phoenix) was analyzed using two complementary LC-MS/MS (Liquid Chromatography - Tandem Quadrupole Mass Spectrometry) methods. These five cultivars belong to fungus-resistant grape varieties (PIWI) and little is known about their phenolic profile, although phenolics such as phytoalexins have been recognized, playing a key role in the resistance mechanism. A triple quadruple mass spectrometer detector was used for both identification and quantification, and analytical tools from untargeted metabolomics were applied to check and control the quality of the results. Specifically, biological, technical, and instrumental replications were included in order to study the variability at different levels. The results allowed tuning of the sampling protocol and provided a rich phenolic metabolite profile for the aforementioned PIWI varieties, as compared with Vitis vinifera cultivars, especially in the class of stilbenes.
采用两种互补的液相色谱 - 串联四极杆质谱法（LC-MS/MS），对在意大利种植的葡萄浆果（赤霞珠柯蒂斯、约翰尼特、索拉里斯、凤凰和丽晶）以及在德国种植的葡萄浆果（丽晶和凤凰）的酚类成分进行了分析。这五个品种属于抗真菌葡萄品种（PIWI），尽管植保素等酚类物质已被确认在抗性机制中发挥关键作用，但对它们的酚类特征却知之甚少。使用三重四极杆质谱仪检测器进行鉴定和定量，并应用非靶向代谢组学的分析工具来检查和控制结果的质量。具体而言，纳入了生物学、技术和仪器重复，以研究不同层面的变异性。结果有助于调整采样方案，并为上述PIWI品种提供了丰富的酚类代谢物图谱，与酿酒葡萄品种相比，尤其是在芪类化合物类别中。
