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采用液相色谱-光电二极管阵列-电喷雾电离-四极杆飞行时间质谱法对隆多和丽晶(欧亚种葡萄)两个种间红色杂交品种的(多)酚类成分进行表征分析。

Characterisation of (poly)phenolic constituents of two interspecific red hybrids of Rondo and Regent (Vitis vinifera) by LC-PDA-ESI-MS QTof.

作者信息

Wojdyło Aneta, Samoticha Justyna, Nowicka Paulina, Chmielewska Joanna

机构信息

Wrocław University of Environmental and Life Sciences, The Faculty of Biotechnology and Food Science, Department of Fruit, Vegetable and Nutraceutical Plant Technology, 37 Chełmońskiego Street, 51-630 Wrocław, Poland.

Wrocław University of Environmental and Life Sciences, The Faculty of Biotechnology and Food Science, Department of Fruit, Vegetable and Nutraceutical Plant Technology, 37 Chełmońskiego Street, 51-630 Wrocław, Poland.

出版信息

Food Chem. 2018 Jan 15;239:94-101. doi: 10.1016/j.foodchem.2017.06.077. Epub 2017 Jun 17.

DOI:10.1016/j.foodchem.2017.06.077
PMID:28873656
Abstract

The aim of this study was to identify and compare phenolic acids, flavan-3-ols, flavonols, and anthocyanins in two the most popular interspecific hybrids of red grapes, Rondo and Regent, nowadays very popular in red wine production in Poland. The phenolic profiles of these hybrids have not yet been reported. Thirty-three phenolic compounds, including 2 flavan-3-ols, 3 phenolic acids, 5 flavonols, and 23 anthocyanins, were determined in the examined samples using the ultra-performance liquid chromatography photodiode detector-quadrupole/time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS) method. Major differences were found in the phenolic profiles of investigated cultivars. The Regent hybrid exhibited the highest total phenolics content (27029.75mg/kg dry matter) but Rondo was characterized by the highest concentration of anthocyanins (19342.36mg/kgdm). The dominant fraction was anthocyanin compounds, especially acetylated>diglucosylated forms than glucosylated ones. This data represents valuable information that may be useful for oenological practices and to valorise these varieties as sources of bioactive compounds.

摘要

本研究的目的是鉴定和比较两种最受欢迎的红葡萄种间杂交品种隆多(Rondo)和丽晶(Regent)中的酚酸、黄烷-3-醇、黄酮醇和花青素,这两个品种目前在波兰的红酒生产中非常受欢迎。尚未有关于这些杂交品种酚类物质概况的报道。使用超高效液相色谱光电二极管检测器-四极杆/飞行时间质谱(UPLC-PDA-Q/TOF-MS)方法,在检测样品中测定了33种酚类化合物,包括2种黄烷-3-醇、3种酚酸、5种黄酮醇和23种花青素。在所研究的品种的酚类物质概况中发现了主要差异。丽晶杂交品种的总酚含量最高(27029.75mg/kg干物质),但隆多的特点是花青素浓度最高(19342.36mg/kg干物质)。主要成分是花青素化合物,尤其是乙酰化>双葡萄糖基化形式,而非葡萄糖基化形式。这些数据代表了有价值的信息,可能对酿酒实践有用,并有助于将这些品种作为生物活性化合物的来源加以利用。

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