Maldini Mariateresa, Maksoud Salwa A, Natella Fausta, Montoro Paola, Petretto Giacomo Luigi, Foddai Marzia, De Nicola Gina Rosalinda, Chessa Mario, Pintore Giorgio
University of Sassari, Department of Chemistry and Pharmacy via F. Muroni, 23/b, 07100, Sassari, Italy.
J Mass Spectrom. 2014 Sep;49(9):900-10. doi: 10.1002/jms.3437.
Moringa oleifera is a medicinal plant and an excellent dietary source of micronutrients (vitamins and minerals) and health-promoting phytochemicals (phenolic compounds, glucosinolates and isothiocyanates). Glucosinolates and isothiocyanates are known to possess anti-carcinogenic and antioxidant effects and have attracted great interest from both toxicological and pharmacological points of view, as they are able to induce phase 2 detoxification enzymes and to inhibit phase 1 activation enzymes. Phenolic compounds possess antioxidant properties and may exert a preventative effect in regards to the development of chronic degenerative diseases. The aim of this work was to assess the profile and the level of bioactive compounds in all parts of M. oleifera seedlings, by using different MS approaches. First, flow injection electrospray ionization mass spectrometry (FI-ESI-MS) fingerprinting techniques and chemometrics (PCA) were used to achieve the characterization of the different plant's organs in terms of profile of phenolic compounds and glucosinolates. Second, LC-MS and LC-MS/MS qualitative and quantitative methods were used for the identification and/or determination of phenolics and glucosinolates in M. oleifera.
辣木是一种药用植物，也是微量营养素（维生素和矿物质）以及具有促进健康作用的植物化学物质（酚类化合物、硫代葡萄糖苷和异硫氰酸盐）的优质膳食来源。硫代葡萄糖苷和异硫氰酸盐具有抗癌和抗氧化作用，从毒理学和药理学角度都引起了极大关注，因为它们能够诱导Ⅱ相解毒酶并抑制Ⅰ相激活酶。酚类化合物具有抗氧化特性，可能对慢性退行性疾病的发展起到预防作用。这项工作的目的是通过使用不同的质谱方法评估辣木幼苗各部分生物活性化合物的概况和水平。首先，采用流动注射电喷雾电离质谱（FI-ESI-MS）指纹图谱技术和化学计量学（PCA），根据酚类化合物和硫代葡萄糖苷的概况对不同植物器官进行表征。其次，使用液相色谱-质谱联用（LC-MS）和液相色谱-串联质谱联用（LC-MS/MS）的定性和定量方法来鉴定和/或测定辣木中的酚类化合物和硫代葡萄糖苷。
