Burgess R M, Sutton J G, Werrett D J
Central Research Establishment, Home Office Forensic Science Service, Reading, Berkshire, U.K.
Forensic Sci Int. 1987 Oct-Nov;35(2-3):213-8. doi: 10.1016/0379-0738(87)90058-2.
The isoelectric focusing patterns of red cell phosphoglucomutase (PGM1) separated on Ampholine, Ampholine/separator and rehydratable immobilised pH gradients have been compared. The sharpest and most intense zymograms have been observed on immobilised pH gradients provided that Ampholine was added during the rehydration of the gel. The addition of ampholine in the rehydration of Immobiline plates has been shown to improve the sharpness and intensity of the zymogram obtained and the potential of immobilised pH gradients for PGM typing has been demonstrated.
比较了在两性电解质、两性电解质/分离剂和可再水化固定化pH梯度上分离的红细胞磷酸葡萄糖变位酶（PGM1）的等电聚焦图谱。在凝胶再水化过程中加入两性电解质的情况下，在固定化pH梯度上观察到了最清晰、最强的酶谱图。已证明在Immobiline板再水化过程中添加两性电解质可提高所得酶谱图的清晰度和强度，并证明了固定化pH梯度用于PGM分型的潜力。
