Lv Chong-Ning, Fan Li, Wang Jing, Qin Ru-Lan, Xu Tan-Ye, Lei Tian-Li, Lu Jin-Cai
a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
J Asian Nat Prod Res. 2015;17(2):132-7. doi: 10.1080/10286020.2014.983091. Epub 2014 Dec 8.
Two new triterpenoid saponins were isolated from the 70% ethanol extract of the rhizome of Anemone amurensis, they are oleanolic acid 28-O-β-d-glucopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 4)-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl ester (1) and 23,27-dihydroxy oleanolic acid 3-O-α-l-arabinopyranoside (2). The structures of 1 and 2 were elucidated on the basis of chemical and spectral analysis, including 1D and 2D NMR data and HR-ESI-MS. Compounds 1 and 2 were tested for cytotoxicities against three human cancer cell lines (A549, Hep-G2, and MCF-7). Compound 1 showed potent cytotoxicity with IC50 values of 34.76, 41.17, and 28.92 μM, respectively, while compound 2 with IC50>100 μM.
从毛茛的根茎70%乙醇提取物中分离得到两种新的三萜皂苷，它们分别是齐墩果酸28 - O -β - d - 吡喃葡萄糖基 -（1→3）-α - l - 吡喃鼠李糖基 -（1→4）-β - d - 吡喃葡萄糖基 -（1→6）-β - d - 吡喃葡萄糖基酯（1）和23,27 - 二羟基齐墩果酸3 - O -α - l - 阿拉伯吡喃糖苷（2）。通过化学和光谱分析，包括一维和二维核磁共振数据以及高分辨电喷雾电离质谱，阐明了化合物1和2的结构。对化合物1和2针对三种人类癌细胞系（A549、Hep - G2和MCF - 7）进行了细胞毒性测试。化合物1显示出较强的细胞毒性，其IC50值分别为34.76、41.17和28.92 μM，而化合物2的IC50>100 μM。
