Chen Zhibao, Li Xinran, Wu Xiuping, Wang Wei, Wang Wendong, Xin Mingxun, Shen Fengge, Liu Lihui, Liang Junchao, Li Lei, Yu Lu
College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China.
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
Iran J Pharm Res. 2014 Spring;13(2):567-73.
The aim of this investigation was to assess the in-vitro interaction of two antifungal agents, econazole-nitrate and chelerythrine, against ten fluconazole-resistant clinical isolates and one ATCC type strain 10231 of Candida albicans. The checkerboard microdilution method was performed according to the recommendations of the National Committee for Clinical Laboratory Standards, and the results were determined by visual examination. The interaction intensity was tested in all isolates using the fractional inhibitory concentration index (FICI). These experiments showed synergism between econazole-nitrate and chelerythrine in antifungal activity against C. albicans, and no antagonistic activity was observed in any of the strains tested. Moreover, time-kill curves were performed with selected strains to confirm the positive interactions. The similarity between the results of the FICI values and the time-kill curves revealed that chelerythrine greatly enhances the antifungal effects of econazole-nitrate against isolates of C. albicans. This synergistic effect may markedly reduce the dose of econazole-nitrate required to treat candidiasis, thereby decreasing the econazole-nitrate toxic side effects. This novel synergism might provide a potential combination treatment against fungal infections.
本研究的目的是评估两种抗真菌剂，即硝酸益康唑和白屈菜红碱，对10株耐氟康唑的白色念珠菌临床分离株和1株美国典型培养物保藏中心（ATCC）10231型白色念珠菌菌株的体外相互作用。棋盘微量稀释法是根据美国国家临床实验室标准委员会的建议进行的，结果通过目视检查确定。使用分数抑菌浓度指数（FICI）在所有分离株中测试相互作用强度。这些实验表明，硝酸益康唑和白屈菜红碱在抗白色念珠菌的抗真菌活性方面具有协同作用，在所测试的任何菌株中均未观察到拮抗活性。此外，对选定菌株进行了时间-杀菌曲线实验以确认阳性相互作用。FICI值结果与时间-杀菌曲线之间的相似性表明，白屈菜红碱可大大增强硝酸益康唑对白色念珠菌分离株的抗真菌作用。这种协同作用可能会显著降低治疗念珠菌病所需的硝酸益康唑剂量，从而减少硝酸益康唑的毒副作用。这种新型协同作用可能为真菌感染提供一种潜在的联合治疗方法。
