γ 射线辐照对大麦（Hordeum vulgare）β-葡聚糖结构和营养潜力的影响。
Effect of γ-irradiation on structure and nutraceutical potential of β-D-glucan from barley (Hordeum vulgare).
机构信息
Department of Food Science and Technology, University of Kashmir, India.
Department of Food Science and Technology, University of Kashmir, India.
出版信息
Int J Biol Macromol. 2015 Jan;72:1168-75. doi: 10.1016/j.ijbiomac.2014.08.056. Epub 2014 Sep 17.
This paper reports the characterization and potential antioxidant activity of β-D-glucan isolated from barley treated with γ-rays. The β-D-glucan was irradiated with 0, 2, 4 and 8 kGy by gamma ray. The samples were characterized by Fourier transform-infrared spectroscopy, gel permeation chromatography (GPC) and quantitative estimation by Megazyme β-D-glucan assay kit. The average molecular weight of non-irradiated β-D-glucan was 177 kDa that decreased to 79 kDa at 8 kGy. Antioxidant activity was evaluated by five complementary assays including DPPH, lipid peroxidation, reducing power, metal chelating ability and oxidative DNA damage assays. Further, the antiproliferative potential of irradiated β-D-glucan was tested against three human cancer cell lines including Colo-205, T47D and MCF7 using MTT assay. Irradiated β-D-glucan exhibited dose dependent cancer cell growth inhibition. In conclusion, the present study demonstrates that irradiation leads to the formation of low molecular weight β-D-glucan with enhanced antioxidant and antiproliferative activities.
本文报道了γ射线处理大麦中提取的β-D-葡聚糖的特性和潜在抗氧化活性。β-D-葡聚糖用 0、2、4 和 8 kGy 的γ射线辐照。采用傅里叶变换红外光谱、凝胶渗透色谱（GPC）和 Megazyme β-D-葡聚糖试剂盒定量分析对样品进行了表征。未经辐照的β-D-葡聚糖的平均分子量为 177 kDa，在 8 kGy 时降至 79 kDa。采用 DPPH、脂质过氧化、还原能力、金属螯合能力和氧化 DNA 损伤测定等五种互补测定法评估了抗氧化活性。进一步，采用 MTT 测定法，用三种人癌细胞系（包括 Colo-205、T47D 和 MCF7）测试了辐照β-D-葡聚糖的增殖抑制活性。辐照β-D-葡聚糖表现出剂量依赖性的癌细胞生长抑制。总之，本研究表明辐照导致形成具有增强的抗氧化和增殖抑制活性的低分子量β-D-葡聚糖。
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