多糖的分离、纯化、结构鉴定及抗氧化活性。
The Separation, Purification, Structure Identification, and Antioxidant Activity of Polysaccharides.
机构信息
School of Life Science, Shanxi University, Taiyuan 030006, China.
Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan 030006, China.
出版信息
Molecules. 2023 Sep 6;28(18):6468. doi: 10.3390/molecules28186468.
In order to investigate the antioxidant activity of polysaccharides, the physicochemical characteristics of purified polysaccharides (EUP, consisting of two fractions, EUP1 and EUP2) were investigated using UV spectrophotometry, high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), and Fourier transform infrared spectroscopy (FT-IR). This revealed that EUP1 and EUP2 were acidic polysaccharides with an average molecular weight (MW) of 63 and 38 kDa, respectively. EUP1 mainly consisted of L-rhamnose and D-galactose in a molar ratio of 2.05:1, and EUP2 consisted of D-mannose, L-rhamnose, D-galactose, and D-arabinose in a molar ratio of 2.06:1:2.78:1. Furthermore, EUP exhibited considerable antioxidant potential for scavenging hydroxyl, superoxide anion, DPPH, and ABTS radicals. Therefore, EUP can be developed as a potential antioxidant for the functional food or pharmaceutical field.
为了研究多糖的抗氧化活性，使用紫外分光光度法、高效液相色谱法（HPLC）、高效凝胶渗透色谱法（HPGPC）和傅里叶变换红外光谱法（FT-IR）对纯化多糖（EUP，由两个部分组成，EUP1 和 EUP2）的理化特性进行了研究。结果表明，EUP1 和 EUP2 是酸性多糖，其平均分子量（MW）分别为 63 和 38 kDa。EUP1 主要由 L-岩藻糖和 D-半乳糖组成，摩尔比为 2.05:1，EUP2 由 D-甘露糖、L-岩藻糖、D-半乳糖和 D-阿拉伯糖组成，摩尔比为 2.06:1:2.78:1。此外，EUP 对清除羟基、超氧阴离子、DPPH 和 ABTS 自由基具有相当大的抗氧化潜力。因此，EUP 可以开发为功能性食品或制药领域的潜在抗氧化剂。
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