Wang Danyang, Zhang Ling, Li Fang, Xu Shuai, Chen Jihua
Hua Xi Kou Qiang Yi Xue Za Zhi. 2014 Aug;32(4):394-9. doi: 10.7518/hxkq.2014.04.018.
To study the types of matrix metalloproteinases (MMPs) involved in dentin bonding interface degradation.
Dentin slices were prepared and treated with two adhesive systems (Single Bond 2 or Clearfil S3 Bond). The dentin surface was bonded with composite resin. All specimens were immersed in sterile artificial saliva for 0 or 6 months, and their micro-shear bond strength (muSBS) were measured. The fracture modes were observed through field emission scanning electron microscope (FE-SEM). Dentin slices with 4 mm x 3 mm x 1 mm dimensions were prepared. The slices were divided into three groups according to the treatment modes (negative control, Single Bond 2, and Clearfil S3 Bond). All specimens were stored in sterile artificial saliva for 0 or 6 months. The concentrations of MMP-1, -2, -3, -8, and -9 of each group were detected through fluorescent microsphere immunoassay.
The muSBS of both adhesive systems significantly decreased after storage aging. Significant differences in failure modes within the four groups tested in this study were observed. Compared with the negative control, the concentrations of MMP-1 and MMP-3 in different adhesive groups showed no significant difference after storage aging. However, the concentrations of MMP-2, -8, and -9 in Single Bond 2 group and the concentrations of MMP-8 and -9 in Clearfil S3 Bond group significantly decreased after 6 months of storage aging.
Significant degradation occur in the dentin bonding interface of both adhesive groups under 6 months aging challenge. The concentrations ofdentinal MMP-2, -8, and -9 significantly decrease after treatment with adhesives and aging, indicating that these MMPs have an important function in dentin bonding interface degradation.
研究参与牙本质粘结界面降解的基质金属蛋白酶（MMPs）类型。
制备牙本质切片，并用两种粘结系统（Single Bond 2或Clearfil S3 Bond）进行处理。将牙本质表面与复合树脂粘结。所有标本浸入无菌人工唾液中0或6个月，测量其微剪切粘结强度（muSBS）。通过场发射扫描电子显微镜（FE-SEM）观察断裂模式。制备尺寸为4mm×3mm×1mm的牙本质切片。根据处理方式将切片分为三组（阴性对照、Single Bond 2和Clearfil S3 Bond）。所有标本在无菌人工唾液中保存0或6个月。通过荧光微球免疫测定法检测每组MMP-1、-2、-3、-8和-9的浓度。
储存老化后，两种粘结系统的muSBS均显著降低。在本研究测试的四组中观察到失效模式存在显著差异。与阴性对照相比，储存老化后不同粘结组中MMP-1和MMP-3的浓度无显著差异。然而，Single Bond 2组中MMP-2、-8和-9的浓度以及Clearfil S3 Bond组中MMP-8和-9的浓度在储存老化6个月后显著降低。
在6个月的老化挑战下，两个粘结组的牙本质粘结界面均发生显著降解。用粘结剂处理并老化后，牙本质MMP-2、-8和-9的浓度显著降低，表明这些MMPs在牙本质粘结界面降解中起重要作用。
