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靶向肝细胞癌中与脂筏相关的Akt信号通路。

Targeting the raft-associated Akt signaling in hepatocellular carcinoma.

作者信息

Liu Yuan, Lv Ji-Yun, Shi Jian-Fei, Yang Mei, Liu Shu-Hong, Li Zhi-Wei, Wang Hong-Bo, Zhang Shao-Geng, Liu Zhen-Wen, Ding Jin-Biao, Xu Dong-Ping, Zhao Jing-Min

机构信息

Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.

First Hepatobiliary Surgery Center, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.

出版信息

Biomed Res Int. 2014;2014:836025. doi: 10.1155/2014/836025. Epub 2014 Aug 27.

DOI:10.1155/2014/836025
PMID:25243186
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4163477/
Abstract

Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 90 human hepatocellular carcinoma (HCC) and adjacent noncancerous tissues (ANT) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 overexpression was found in HCC tissues compared to ANT and was confirmed by immunohistochemistry. Raft-associated Akt signaling pathway components involved in the regulation of cell survival were altered by western blotting in HCC microdomain-enriched subcellular fractions purified from paired HCC and ANT samples. Our results demonstrated that the activity of raft-associated but not total membrane Akt determines its cellular functions. Lipid rafts differ in different types of tissues, which allows for the possibility of tissue-type-specific targeting for cell survival.

摘要

小窝蛋白-1和小窝蛋白-1被认为是脂筏的标志物，脂筏可被视为跨膜蛋白靶向运输的分选平台，并参与信号转导、细胞黏附、脂质/蛋白质分选以及人类癌症等基本细胞事件。我们通过SDS-PAGE和用特异性抗体进行免疫印迹，研究了90例人肝细胞癌（HCC）和癌旁非癌组织（ANT）样本中小窝蛋白-1和小窝蛋白-1的表达。与ANT相比，在HCC组织中发现小窝蛋白-1和小窝蛋白-1明显过表达，并通过免疫组织化学得到证实。通过蛋白质印迹法，在从配对的HCC和ANT样本中纯化的富含HCC微区的亚细胞组分中，参与细胞存活调节的脂筏相关Akt信号通路成分发生了改变。我们的结果表明，脂筏相关而非总膜Akt的活性决定了其细胞功能。脂筏在不同类型的组织中有所不同，这使得针对细胞存活进行组织类型特异性靶向成为可能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/174240ec1dae/BMRI2014-836025.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/03bf34213bd5/BMRI2014-836025.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/94b70505946b/BMRI2014-836025.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/afce8ecc39f7/BMRI2014-836025.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/12a6341bd7d7/BMRI2014-836025.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/174240ec1dae/BMRI2014-836025.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/03bf34213bd5/BMRI2014-836025.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/94b70505946b/BMRI2014-836025.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/afce8ecc39f7/BMRI2014-836025.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/12a6341bd7d7/BMRI2014-836025.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3729/4163477/174240ec1dae/BMRI2014-836025.005.jpg

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Front Pharmacol. 2018 Nov 13;9:1223. doi: 10.3389/fphar.2018.01223. eCollection 2018.
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