Kumar K Senthil, Babu Aravindh, Sundarapandian G, Roy Parimal, Thangavelu A, Kumar K Siva, Arumugam R, Chandran N D J, Muniraju Murali, Mahapatra Mana, Banyard Ashley C, Manohar B Murali, Parida Satya
Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.
National Institute for Animal Biotechnology, Hyderabad 500049, India.
Vet Microbiol. 2014 Nov 7;174(1-2):39-49. doi: 10.1016/j.vetmic.2014.08.031. Epub 2014 Sep 6.
Peste des petits ruminants is responsible for an economically important plague of small ruminants that is endemic across much of the developing world. Here we describe the detection and characterisation of a PPR virus from a recent outbreak in Tamil Nadu, India. We demonstrate the isolation of PPR virus from rectal swab and highlight the potential spread of disease to in-contact animals through faecal materials and use of faecal material as non-invasive method of sampling for susceptible wild ruminants. Finally we have performed a comprehensive 'multi-gene' assessment of lineage IV isolates of PPRV utilising sequence data from our study and publically available partial N, partial F and partial H gene data. We describe the effects of grouping PPRV isolates utilising different gene loci and conclude that the variable part of N gene at C terminus gives the best phylogenetic assessment of PPRV isolates with isolates generally clustering according to geographical isolation. This assessment highlights the importance of careful gene targeting with RT-PCR to enable thorough phylogenetic analysis.
小反刍兽疫是一种对小反刍动物具有重要经济影响的疫病，在许多发展中国家呈地方流行。在此，我们描述了从印度泰米尔纳德邦近期一次疫情中检测和鉴定的一株小反刍兽疫病毒。我们展示了从小肠拭子中分离出小反刍兽疫病毒，并强调了疾病通过粪便材料向接触动物传播的可能性，以及将粪便材料用作易感野生反刍动物非侵入性采样方法的用途。最后，我们利用本研究的序列数据以及公开可得的部分N基因、部分F基因和部分H基因数据，对小反刍兽疫病毒IV系分离株进行了全面的“多基因”评估。我们描述了利用不同基因位点对小反刍兽疫病毒分离株进行分组的效果，并得出结论，N基因C末端的可变部分对小反刍兽疫病毒分离株的系统发育评估最佳，分离株通常根据地理隔离进行聚类。这一评估突出了通过逆转录聚合酶链反应进行仔细基因靶向以实现全面系统发育分析的重要性。
