Molecular Microbiology Laboratory, Veterinary Control Institute, 42090, Konya, Turkey.
Department of Molecular Biology and Genetics, Faculty of Science, Necmettin Erbakan University, 42090, Konya, Turkey.
Arch Virol. 2022 Mar;167(3):941-946. doi: 10.1007/s00705-022-05386-x. Epub 2022 Feb 11.
Peste des petits ruminants (PPR) mostly affects small ruminants. Vaccination with attenuated vaccines derived from PPR virus (PPRV) provides successful protection against the disease. However, current molecular methods are unable to distinguish vaccine strains from field strains. In this study, we used an EvaGreen-based high-resolution melting (HRM) assay to differentiate a PPRV vaccine strain (Nigeria 75/1) from Turkish field isolates of lineage IV based on melting peaks and melting profiles.
小反刍兽疫(PPR)主要感染小反刍动物。用弱毒疫苗对小反刍兽疫病毒(PPRV)进行免疫接种能成功预防该疾病。然而,目前的分子方法无法区分疫苗株和野毒株。在本研究中,我们使用 EvaGreen 基高分辨率熔解(HRM)分析基于熔解峰和熔解曲线,从小反刍兽疫病毒尼日利亚 75/1 疫苗株和土耳其 IV 谱系的野毒株中进行区分。