Organic Chemistry Institute and Graduate School Chemistry, Westfälische Wilhelms-Universität Münster, Correnstrasse 40, 48149 Münster (Germany).
Angew Chem Int Ed Engl. 2014 Nov 17;53(47):12946-50. doi: 10.1002/anie.201405849. Epub 2014 Sep 22.
The easy and effective separation of proteins from a mixture is crucial in proteomics. A supramolecular method is described to selectively capture and precipitate one protein from a protein mixture upon application of a magnetic field. A multivalent complex self-assembles in a dilute aqueous solution of three components: magnetic nanoparticles capped with cyclodextrin, non-covalent cross-linkers with an adamantane and a carbohydrate moiety, and lectins. The self-assembled ternary complex is precipitated in a magnetic field and readily redispersed with the aid of a non-ionic surfactant and competitive binding agents. This strategy to purify proteins by supramolecular magnetic precipitation is highly selective and efficient.
从混合物中轻松有效地分离蛋白质在蛋白质组学中至关重要。本文描述了一种超分子方法，通过施加磁场，从蛋白质混合物中选择性地捕获和沉淀一种蛋白质。在含有三种成分的稀水溶液中，通过自组装形成多价配合物：具有环糊精的磁性纳米粒子、具有金刚烷和碳水化合物部分的非共价交联剂以及凝集素。在磁场中沉淀自组装的三元配合物，并在非离子表面活性剂和竞争性结合剂的辅助下，很容易重新分散。通过超分子磁性沉淀来纯化蛋白质的这种策略具有高度的选择性和高效性。
