†Department of Chemistry, Yonsei University, Seoul 120-749 Korea.
‡Department of Veterinary Medicine, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701 Korea.
J Am Chem Soc. 2015 May 13;137(18):5961-8. doi: 10.1021/jacs.5b00592. Epub 2015 May 5.
Dual-modal fluorescent magnetic glyconanoparticles have been prepared and shown to be powerful in probing lectins displayed on pathogenic and mammalian cell surfaces. Blood group H1- and Le(b)-conjugated nanoparticles were found to bind to BabA displaying Helicobacter pylori, and Le(a)- and Le(b)-modified nanoparticles are both recognized by and internalized into DC-SIGN and SIGN-R1 expressing mammalian cells via lectin-mediated endocytosis. In addition, glyconanoparticles block adhesion of H. pylori to mammalian cells, suggesting that they can serve as inhibitors of infection of host cells by this pathogen. It has been also shown that owing to their magnetic properties, glyconanoparticles are useful tools to enrich lectin expressing cells. The combined results indicate that dual-modal glyconanoparticles are biocompatible and that they can be employed in lectin-associated biological studies and biomedical applications.
双模态荧光磁性糖纳米粒子已经被制备出来，并被证明在探测病原体和哺乳动物细胞表面展示的凝集素方面非常有效。已经发现，血型 H1- 和 Le(b)- 结合的纳米粒子与幽门螺杆菌上的 BabA 结合，而 Le(a)- 和 Le(b)- 修饰的纳米粒子都可以被表达 DC-SIGN 和 SIGN-R1 的哺乳动物细胞识别，并通过凝集素介导的内吞作用被内化。此外，糖纳米粒子可以阻止幽门螺杆菌与哺乳动物细胞的黏附，这表明它们可以作为宿主细胞感染这种病原体的抑制剂。此外，由于其磁性，糖纳米粒子是富集表达凝集素的细胞的有用工具。综合结果表明，双模态糖纳米粒子具有生物相容性，可用于凝集素相关的生物学研究和生物医学应用。
