Puré E, Inaba K, Metlay J
Rockefeller University, New York, New York.
J Exp Med. 1988 Aug 1;168(2):795-800. doi: 10.1084/jem.168.2.795.
Although the production of B cell stimulatory factors by cell lines and hybridomas is well established, production of specific lymphokines by normal T cells in response to antigen stimulation has not been analyzed. We have used bioassays and neutralizing mAbs to demonstrate that IL-2, IL-4, and B cell growth factors (BCGF) are produced during primary and secondary MLRs. IL-2 is detected in the first 12 h of both types of MLR. IL-4 and BCGF appear at 24-48 h in the conditioned medium of the primary MLR, and peak by 12 h in the secondary MLR. The amount of IL-4 in the primary response reaches a level that is 10% of that detected in the secondary. In contrast, BCGF production steadily increases over time in the primary MLR, and maximal production is equivalent to that made in the secondary response. Allogeneic dendritic cells and anti-Ig-activated B blasts both stimulated lymphokine production in the primary MLR, whereas small B cells were weak. In the secondary MLR, all three cell populations stimulated the production of IL-2, IL-4, and BCGF. Therefore, the release of several defined B cell stimulating factors can be detected in the conditioned media of responding primary T lymphocytes.
虽然细胞系和杂交瘤产生B细胞刺激因子的现象已得到充分证实，但正常T细胞在抗原刺激下产生特定淋巴因子的情况尚未得到分析。我们已使用生物测定法和中和单克隆抗体来证明，白细胞介素-2（IL-2）、白细胞介素-4（IL-4）和B细胞生长因子（BCGF）在初次和二次混合淋巴细胞反应（MLR）过程中产生。在两种类型的MLR的最初12小时内均可检测到IL-2。IL-4和BCGF在初次MLR的条件培养基中于24 - 48小时出现，并在二次MLR中于12小时达到峰值。初次反应中IL-4的量达到二次反应中检测量的10%。相比之下，在初次MLR中BCGF的产生随时间稳步增加，最大产量与二次反应中的产量相当。同种异体树突状细胞和抗Ig激活的B母细胞在初次MLR中均刺激淋巴因子产生，而小B细胞的刺激作用较弱。在二次MLR中，所有三种细胞群体均刺激IL-2、IL-4和BCGF的产生。因此，在反应性原代T淋巴细胞的条件培养基中可检测到几种特定的B细胞刺激因子的释放。
