Leite Vanessa Maria Fagundes, Pinheiro Juliana Barchelli, Pisani Marina Xavier, Watanabe Evandro, de Souza Raphael Freitas, Paranhos Helena de Freitas Oliveira, Lovato-Silva Cláudia Helena
Department of Dental Materials and Prosthesis, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Braz Dent J. 2014;25(3):191-6. doi: 10.1590/0103-6440201302382.
This study evaluated the antimicrobial activity of a Ricinus communis-based experimental dentifrice for denture hygiene against the following standard strains: Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Enterococcus faecalis, Candida albicans and Candida glabrata. The minimum inhibitory concentration (MIC) assay was performed with R. communis in pure oil at 2.5%. Only E. coli was not inhibited by R. communis, but the MIC (0.0781%) was effective against the other microorganisms. From these results it was determined the R. communis concentrations for experimental dentifrices, 1, 2, 5 and 10%, which were evaluated by the test-well diffusion in agar. The commercial dentifrices Colgate, Trihydral and Corega Brite were tested for comparative purposes. The diameter of the zones of bacterial growth inhibition produced around the wells was measured (in mm) with a rule under reflected light. Data were analyzed statistically by analysis of variance and Tukey's post-hoc test (α=0.05). Neither the commercial nor the experimental dentifrices were effective against E. coli. The experimental dentifrices containing R. communis at 2, 5 and 10% presented action against S. mutans, S. aureaus and E. faecallis. The experimental dentifrices showed no antimicrobial activity against Candida spp. and E. coli in any of the tested concentrations. Trihydral was the most effective. Comparing the experimental dentifrices, the product with 10% R. communis produced the largest zones of bacterial growth inhibition and had similar antimicrobial activity to the commercial dentifrices, except against S. aureus.
本研究评估了一种基于蓖麻的实验性洁齿剂对义齿卫生的抗菌活性，该洁齿剂针对以下标准菌株：金黄色葡萄球菌、大肠杆菌、变形链球菌、粪肠球菌、白色念珠菌和光滑念珠菌。采用2.5%的纯蓖麻油进行最低抑菌浓度（MIC）测定。只有大肠杆菌未被蓖麻抑制，但MIC（0.0781%）对其他微生物有效。根据这些结果确定了实验性洁齿剂中蓖麻的浓度为1%、2%、5%和10%，并通过琼脂孔扩散试验进行评估。为作比较，对高露洁、Trihydral和Corega Brite等市售洁齿剂进行了测试。在反射光下用直尺测量孔周围产生的细菌生长抑制圈的直径（以毫米为单位）。数据采用方差分析和Tukey事后检验进行统计学分析（α=0.05）。市售和实验性洁齿剂对大肠杆菌均无效。含2%、5%和10%蓖麻的实验性洁齿剂对变形链球菌、金黄色葡萄球菌和粪肠球菌有作用。实验性洁齿剂在任何测试浓度下对念珠菌属和大肠杆菌均无抗菌活性。Trihydral最有效。比较实验性洁齿剂，含10%蓖麻的产品产生的细菌生长抑制圈最大，并具有与市售洁齿剂相似的抗菌活性，但对金黄色葡萄球菌除外。
