Thoen J, Waalen K, Førre O, Kvarnes L, Natvig J B
Institute of Immunology and Rheumatology, Rikshospitalet, Oslo, Norway.
Scand J Rheumatol. 1989;18(2):77-88. doi: 10.3109/03009748909099922.
Mononuclear cells were eluted from synovial membranes of 39 patients with rheumatoid arthritis and 12 patients with juvenile rheumatoid arthritis. A considerable cell loss, about 50% or more, was seen during the various isolation steps. The CD4/CD8 ratio just after enzyme treatment (stage I) was significantly higher than at later stages, i.e. after removal of adherent cells (stage II, p less than 0.05) and after Isopaque Ficoll gradient centrifugation (stage III, p less than 0.01). This indicates a selective loss of CD4+ cells during isolation. In addition, stages I and II had higher CD4/CD8 ratios than peripheral blood of normal controls (p less than 0.01 and p less than 0.03), but not significantly higher than in peripheral blood of patients (p greater than 0.05). The CD4/CD8 ratio in eluted synovial membrane cells did not differ between patients with high and patients with low disease activity (p greater than 0.05). No correlation was found between any of the CD4/CD8 ratios and individual disease activity variables. Furthermore, a laboratory activity index and a disease outcome index were determined for each patient and no correlation was found between these indices and the CD4/CD8 ratios.
从39例类风湿性关节炎患者和12例青少年类风湿性关节炎患者的滑膜中洗脱单核细胞。在各个分离步骤中观察到相当多的细胞损失，约50%或更多。酶处理后（阶段I）的CD4/CD8比值显著高于后期阶段，即在去除贴壁细胞后（阶段II，p<0.05）和在Ficoll分层液梯度离心后（阶段III，p<0.01）。这表明在分离过程中CD4+细胞有选择性损失。此外，阶段I和II的CD4/CD8比值高于正常对照的外周血（p<0.01和p<0.03），但不显著高于患者的外周血（p>0.05）。洗脱的滑膜细胞中的CD4/CD8比值在疾病活动度高的患者和疾病活动度低的患者之间没有差异（p>0.05）。在任何CD4/CD8比值与个体疾病活动变量之间均未发现相关性。此外，为每位患者确定了实验室活动指数和疾病结局指数，并且在这些指数与CD4/CD8比值之间未发现相关性。
