Department of Materia Medica, School of Pharmacology, Fourth Military Medical University, Xi'an, Shaanxi, China.
BMC Complement Altern Med. 2014 Sep 23;14:348. doi: 10.1186/1472-6882-14-348.
The current study was to evaluate the anti-thrombotic effect of alpha-linolenic acid (ALA) which was isolated and purified from Jiaomu in vivo.
The seeds were crushed and subsequently subjected to saponification, acid hydrolysis, gradient freezing, urea inclusion and complexation of silver nitrate to obtain the unsaturated fatty acids. The chemical characteristics of isolated ALA were validated by 1HNMR, 13CNMR and mass spectrometry, and then the anti-thrombotic effect of ALA and its mixture with linoleic acid (1:1) were evaluated in the following experiments.
The alpha-linolenic acid was isolated and purified from Jiaomu through our newly established methods. ALA and its mixture with linoleic acid can prolong the hemorrhage and coagulation time as well as enhanced the survival rate of mice subjected to collagen-adrenaline induced thrombosis. In addition, the thrombosis on A-V bypass and platelet aggregation of rats will be reduced after treated with ALA or its mixture, and the expression level of Akt and PI3K protein decreased 26% and 31%, respectively.
We designed and optimized a very simple and high-yield procedure to isolate ALA and linoleic acid mixture from seeds of Zanthoxylum bungeanum Maxim and demonstrated that such mixture can obtain a good anti-thrombotic effect through the modulation of PI3K/Akt signaling.
本研究旨在评估从花椒中分离和纯化的α-亚麻酸（ALA）的抗血栓作用。
将种子粉碎，然后进行皂化、酸水解、梯度冷冻、尿素包合和硝酸银络合，得到不饱和脂肪酸。通过 1HNMR、13CNMR 和质谱验证分离出的 ALA 的化学特性，然后在以下实验中评估 ALA 及其与亚油酸（1:1）的混合物的抗血栓作用。
通过我们新建立的方法从花椒中分离和纯化出 α-亚麻酸。ALA 及其与亚油酸的混合物可延长出血和凝血时间，并提高胶原-肾上腺素诱导的血栓形成小鼠的存活率。此外，ALA 或其混合物处理可降低大鼠动静脉旁路血栓形成和血小板聚集，Akt 和 PI3K 蛋白表达水平分别降低 26%和 31%。
我们设计并优化了一种非常简单且高产的从花椒种子中分离 ALA 和亚油酸混合物的方法，并证明该混合物可通过调节 PI3K/Akt 信号通路获得良好的抗血栓作用。
