Wang Huimin, Xiang Tingsheng, Wang Yanwei, Song Jinlong, Zhai Yi, Chen Xiaorong, Li Yanting, Zhao Bingqiang, Zhao Bin, Ruan Zhiyong
Earth Environmental and Water Resources College, Yangtze University, Jingzhou 434023, PR China.
Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2014 Dec;64(Pt 12):4168-4172. doi: 10.1099/ijs.0.061119-0. Epub 2014 Sep 24.
A Gram-staining-positive, rod-shaped, aerobic bacterium, designated strain LAM0410(T), was isolated from an oil-contaminated water sample from the Dagang Oilfield, PR China. The temperature and pH ranges for growth were 4 °C to 50 °C and pH 4.4 to pH 12.0, respectively. The strain did not need NaCl for growth, but could tolerate a concentration of up to 12 % (w/v). Cell wall hydrolysates from the isolate showed that the diamino acid was ornithine. The cell wall sugars contained ribose and galactose. The glycan moiety of the cell wall contained N-glycolyl residues. The major respiratory quinones were MK-10, MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and nine unknown glycolipids. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium sediminis MCCC 1A06153(T) and Microbacterium murale DSM 22178(T) with 97.5 % and 97.4 % sequence similarities, respectively; while the DNA-DNA hybridization values were 33.1±3.4 % and 21.8±1.6 %, respectively. The DNA G+C content was 67.4 mol%, as determined by the Tm method. Based on its phenotypic and genotypic properties, strain LAM0410(T) represents a novel species of the genus Microbacterium, for which the name Microbacterium petrolearium sp. nov. is proposed; the type strain is LAM0410(T) ( = ACCC 00719(T) = JCM 19612(T)).
