钩端螺旋体属种在钩端螺旋体 Vanaporn Wuthiekanun 琼脂中的维持。
Maintenance of leptospira species in leptospira Vanaporn Wuthiekanun agar.
机构信息
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Nuffield Department of Medicine, University of Oxford, Old Road Campus, Headington, Oxford, United Kingdom.
出版信息
J Clin Microbiol. 2014 Dec;52(12):4350-2. doi: 10.1128/JCM.02273-14. Epub 2014 Sep 24.
The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.
在液体或半固体培养基中维持钩端螺旋体属（Leptospira）物种既耗时又容易受到污染，因为需要进行常规传代和暗场显微镜检查。使用钩端螺旋体属（Leptospira）Vanaporn Wuthiekanun（LVW）琼脂，我们无需传代即可在 12 个月内维持 100 株致病性钩端螺旋体属分离株，并通过肉眼确认所有分离株的活力。
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