Tanaka K, Kohno S, Miyazaki T, Miyazaki H, Mitsutake K, Maesaki S, Kaku M, Koga H
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Mycopathologia. 1994 Sep;127(3):131-4. doi: 10.1007/BF01102911.
A latex agglutination test for cryptococcal antigen, the Eiken Latex test (Eiken, Tokyo, Japan), was compared with a monoclonal antibody-based agglutination assay, Pastorex Cryptococcus (Diagnostics Pasteur, Marneur-la-Coquette, France). In a murine model of disseminated cryptococcosis, the kinetics of the antigen titers by the Eiken Latex were similar to those by the Pastorex Cryptococcus, but sensitivity was much higher. In HIV-negative patients with pulmonary cryptococcosis, a cryptococcal antigen was detected in 6 of 10 patients by the Eiken Latex test and in only 3 of those patients by the Pastorex Cryptococcus. The results indicate that the Eiken Latex is more sensitive for the detection of the cryptococcal antigen, even in non-disseminated cryptococcosis. The sensitivity and specificity of the Eiken Latex were examined using 195 sera from 25 patients with pulmonary cryptococcosis and 170 patients with non-cryptococcosis. The cutoff value of > or = 1:8 showed a sensitivity of 76% (19/25) and a specificity of 98.9% (168/170).
将用于检测隐球菌抗原的乳胶凝集试验（艾肯乳胶试验，日本东京艾肯公司生产）与基于单克隆抗体的凝集试验（法国马尔讷拉科凯特巴斯德诊断公司生产的帕斯托雷克斯隐球菌试验）进行了比较。在播散性隐球菌病的小鼠模型中，艾肯乳胶试验检测抗原滴度的动力学与帕斯托雷克斯隐球菌试验相似，但灵敏度要高得多。在患有肺隐球菌病的HIV阴性患者中，10名患者中有6名通过艾肯乳胶试验检测到隐球菌抗原，而通过帕斯托雷克斯隐球菌试验仅在其中3名患者中检测到。结果表明，即使在非播散性隐球菌病中，艾肯乳胶试验在检测隐球菌抗原方面也更敏感。使用来自25例肺隐球菌病患者和170例非隐球菌病患者的195份血清检测了艾肯乳胶试验的灵敏度和特异性。≥1:8的临界值显示灵敏度为76%（19/25），特异性为98.9%（168/170）。
