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筛选具有调节葡萄糖摄取和脂肪积累的生物活性代谢产物的植物提取物。

Screening for bioactive metabolites in plant extracts modulating glucose uptake and fat accumulation.

机构信息

Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.

Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark.

出版信息

Evid Based Complement Alternat Med. 2014;2014:156398. doi: 10.1155/2014/156398. Epub 2014 Aug 28.

DOI:10.1155/2014/156398
PMID:25254050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4164421/
Abstract

Dichloromethane and methanol extracts of seven different food and medicinal plants were tested in a screening platform for identification of extracts with potential bioactivity related to insulin-dependent glucose uptake and fat accumulation. The screening platform included a series of in vitro bioassays, peroxisome proliferator-activated receptor (PPAR) γ-mediated transactivation, adipocyte differentiation of 3T3-L1 cell cultures, and glucose uptake in both 3T3-L1 adipocytes and primary porcine myotubes, as well as one in vivo bioassay, fat accumulation in the nematode Caenorhabditis elegans. We found that dichloromethane extracts of aerial parts of golden root (Rhodiola rosea) and common elder (Sambucus nigra) as well as the dichloromethane extracts of thyme (Thymus vulgaris) and carrot (Daucus carota) were able to stimulate insulin-dependent glucose uptake in both adipocytes and myotubes while weekly activating PPARγ without promoting adipocyte differentiation. In addition, these extracts were able to decrease fat accumulation in C. elegans. Methanol extracts of summer savory (Satureja hortensis), common elder, and broccoli (Brassica oleracea) enhanced glucose uptake in myotubes but were not able to activate PPARγ, indicating a PPARγ-independent effect on glucose uptake.

摘要

七种不同的食品和药用植物的二氯甲烷和甲醇提取物在一个筛选平台中进行了测试，以鉴定与胰岛素依赖性葡萄糖摄取和脂肪积累相关的潜在生物活性提取物。筛选平台包括一系列体外生物测定、过氧化物酶体增殖物激活受体 (PPAR)γ 介导的转录激活、3T3-L1 细胞培养物中的脂肪细胞分化、3T3-L1 脂肪细胞和原代猪肌管中的葡萄糖摄取，以及一个体内生物测定，秀丽隐杆线虫 (Caenorhabditis elegans) 中的脂肪积累。我们发现，金色根（Rhodiola rosea）和普通接骨木（Sambucus nigra）地上部分的二氯甲烷提取物以及百里香（Thymus vulgaris）和胡萝卜（Daucus carota）的二氯甲烷提取物能够刺激脂肪细胞和肌管中的胰岛素依赖性葡萄糖摄取，同时每周激活 PPARγ 而不促进脂肪细胞分化。此外，这些提取物能够减少秀丽隐杆线虫中的脂肪积累。夏季马郁兰（Satureja hortensis）、普通接骨木和西兰花（Brassica oleracea）的甲醇提取物能够增加肌管中的葡萄糖摄取，但不能激活 PPARγ，表明对葡萄糖摄取有 PPARγ 独立的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6d/4164421/77ace5239de2/ECAM2014-156398.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6d/4164421/7f68f385b4af/ECAM2014-156398.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6d/4164421/b9fc61065f96/ECAM2014-156398.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6d/4164421/77ace5239de2/ECAM2014-156398.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6d/4164421/7f68f385b4af/ECAM2014-156398.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6d/4164421/b9fc61065f96/ECAM2014-156398.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6d/4164421/77ace5239de2/ECAM2014-156398.003.jpg

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