Mayorga-Ramos Arianna, Zúñiga-Miranda Johana, Coyago-Cruz Elena, Heredia-Moya Jorge, Guamán-Bautista Jéssica, Guamán Linda P
Centro de Investigación Biomédica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170129, Ecuador.
Carrera de Ingeniería en Biotecnología de los Recursos Naturales, Universidad Politécnica Salesiana, Sede Quito, Campus El Girón, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador.
Antioxidants (Basel). 2025 Mar 27;14(4):394. doi: 10.3390/antiox14040394.
, a fruit-bearing species of the Ericaceae family, has traditionally been used for its potential medicinal properties.
BACKGROUND/OBJECTIVES: This study investigates the phytochemical composition and antimicrobial activity of fruit extract, focusing on its antibacterial, antibiofilm, and antifungal effects.
(Kunth) A.C.Sm. berries (code: 4456, Herbario QUPS-Ecuador) were collected from the cloud forest Montano Alto, Cuenca-Ecuador, and the extract was obtained using an ethanolic-based extraction and chemically characterized. The antibacterial and antifungal activity of the fruit extract was assessed against seven multidrug-resistant bacteria strains and four fungal strains using the microdilution method. The biofilm inhibition potential was evaluated using a microplate assay with the crystal violet staining method. The antioxidant activity was evaluated using DPPH and ABTS assays.
The bioactive compounds showed 853.9 mg phenols/100 g DW, 573.2 mg organic acid/100 g DW, and 21.5 mg C-3-gl/100 g DW of anthocyanins. The antibacterial assays demonstrated significant inhibitory activity against , , , and , with MIC values ranging from 1.25 to 5 mg/mL. Additionally, the biofilm inhibition assays confirmed the potential of extract to disrupt bacterial biofilms, particularly in and . Nevertheless, no significant antifungal activity was observed against spp., suggesting selective antimicrobial properties. Finally, the antioxidant activity was strong (1.62 mmol TE/100 g DW by DPPH and 3.28 mmol TE/100 g DW by ABTS).
These findings indicate that possesses promising antibacterial, antibiofilm, and antioxidant properties, which may be attributed to its phenolic and organic acid composition. Further fractionation and targeted bioassays are required to elucidate the specific bioactive compounds responsible for these effects and explore their potential applications in antimicrobial formulations.
[植物名称]，杜鹃花科的一种结果实的物种，传统上因其潜在的药用特性而被使用。
背景/目的：本研究调查了[植物名称]果实提取物的植物化学成分和抗菌活性，重点关注其抗菌、抗生物膜和抗真菌作用。
从厄瓜多尔昆卡的蒙塔诺阿尔托云雾森林中采集了[植物名称]（Kunth）A.C.Sm.浆果（编号：4456，厄瓜多尔昆卡大学植物标本馆），并使用乙醇基提取法获得提取物并进行化学表征。采用微量稀释法评估果实提取物对七种多重耐药细菌菌株和四种真菌菌株的抗菌和抗真菌活性。使用结晶紫染色法的微孔板测定法评估生物膜抑制潜力。使用DPPH和ABTS测定法评估抗氧化活性。
生物活性化合物显示出853.9毫克酚类/100克干重、573.2毫克有机酸/100克干重和21.5毫克花青素C - 3 - 葡萄糖苷/100克干重。抗菌试验表明对[细菌名称1]、[细菌名称2]、[细菌名称3]和[细菌名称4]具有显著的抑制活性，最低抑菌浓度值范围为1.25至5毫克/毫升。此外，生物膜抑制试验证实了[植物名称]提取物破坏细菌生物膜的潜力，特别是在[细菌名称5]和[细菌名称6]中。然而，对[真菌名称] spp.未观察到显著的抗真菌活性，表明其具有选择性抗菌特性。最后，抗氧化活性很强（DPPH法为1.62毫摩尔TE/100克干重，ABTS法为3.28毫摩尔TE/100克干重）。
这些发现表明[植物名称]具有有前景的抗菌、抗生物膜和抗氧化特性，这可能归因于其酚类和有机酸成分。需要进一步分离和靶向生物测定以阐明负责这些作用的特定生物活性化合物，并探索它们在抗菌制剂中的潜在应用。
