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通过对黄曲霉菌和玉米粒进行全面的 LC-MS/MS 分析来定量分析氧化脂类。

Quantitative profiling of oxylipins through comprehensive LC-MS/MS analysis of Fusarium verticillioides and maize kernels.

机构信息

a Dipartimento di Biologia Ambientale , Università Sapienza , Rome , Italy.

出版信息

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2014;31(12):2026-33. doi: 10.1080/19440049.2014.968810. Epub 2014 Nov 5.

Abstract

Fusarium verticillioides is one of the most important fungal pathogens causing ear and stalk rot in maize, even if frequently asymptomatic, producing a harmful series of compounds named fumonisins. Plant and fungal oxylipins play a crucial role in determining the outcome of the interaction between the pathogen and its host. Moreover, oxylipins result as signals able to modulate the secondary metabolism in fungi. In keeping with this, a novel, quantitative LC-MS/MS method was designed to quantify up to 17 different oxylipins produced by F. verticillioides and maize kernels. By applying this method, we were able to quantify oxylipin production in vitro - F. verticillioides grown into Czapek-Dox/yeast extract medium amended with 0.2% w/v of cracked maize - and in vivo, i.e. during its growth on detached mature maize ears. This study pinpoints the role of oxylipins in a plant pathogen such as F. verticillioides and sets up a novel tool aimed at understanding the role oxylipins play in mycotoxigenic pathogens during their interactions with respective hosts.

摘要

镰刀菌(Fusarium verticillioides)是引起玉米穗腐和茎腐的最重要的真菌病原体之一,即使经常无症状,也会产生一系列名为伏马菌素的有害化合物。植物和真菌的氧化脂类在决定病原体与其宿主相互作用的结果方面起着至关重要的作用。此外,氧化脂类是能够调节真菌次生代谢的信号。因此,设计了一种新的、定量的 LC-MS/MS 方法来定量测定由 F. verticillioides 和玉米籽粒产生的多达 17 种不同的氧化脂类。通过应用该方法,我们能够定量测定体外 F. verticillioides 在添加 0.2%w/v 粉碎玉米的 Czapek-Dox/酵母提取物培养基中的氧化脂类产生情况,以及体内即在其生长于分离的成熟玉米穗上的情况。本研究指出了氧化脂类在 F. verticillioides 等植物病原体中的作用,并建立了一种新的工具,旨在了解氧化脂类在真菌毒素产生性病原体与其各自宿主相互作用过程中所起的作用。

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