Ye Xiaolan, Cao Di, Zhao Xin, Song Fenyun, Huang Qinghua, Fan Guorong, Wu Fuhai
Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China; Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, No. 325 Guohe Road, Shanghai 200433, PR China.
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, No. 325 Guohe Road, Shanghai 200433, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Nov 1;970:108-20. doi: 10.1016/j.jchromb.2014.06.035. Epub 2014 Jul 6.
A method incorporating HPLC-PDA-IT-MS(n) with HPLC-Quadrupole-Orbitrap-MS was developed for the investigation of chemical fingerprint of Citrus reticulate 'Chachi' decoction (CRCD) and metabolic profile of SD rat plasma sample after oral administration of CRCD (1.5 g herb/kg). A total of 27 chemical constituents of CRCD were identified from their MW, UV spectra, MS(n) data and retention behavior by comparing the results with those of the reference standards or literature. And 43 compounds were detected in dosed SD rat plasma samples, including 9 prototypes which were identified as hesperetin, isosinensetin, sinensetin, tetramethyl-O-isoscutellarein, nobiletin, tetramethyl-O-scutellarein, HMF (3,5,6,7,8,3',4'-heptamethoxyflavone), tangeretin and 5-demethylnobiletin and 34 metabolites underwent metabolic process of demethylation, glucuronide conjugation, sulfate conjugation or mixed modes. This is the first research for the metabolic profile of CRCD in SD rats, which could lay a foundation for the further studies of CRC or its formulation.
建立了一种将高效液相色谱-光电二极管阵列-离子阱质谱联用（HPLC-PDA-IT-MS(n)）与高效液相色谱-四极杆-轨道阱质谱联用的方法，用于研究化橘红汤（CRCD）的化学指纹图谱以及SD大鼠口服CRCD（1.5 g生药/kg）后血浆样品的代谢轮廓。通过将结果与参考标准品或文献的结果进行比较，根据分子量、紫外光谱、质谱(n)数据和保留行为，共鉴定出CRCD中的27种化学成分。在给药后的SD大鼠血浆样品中检测到43种化合物，其中包括9种原型成分，分别鉴定为橙皮素、异川陈皮素、川陈皮素、四甲基-O-异黄芩素、诺米林、四甲基-O-黄芩素、HMF（3,5,6,7,8,3',4'-七甲氧基黄酮）、橘皮素和5-去甲基诺米林，还有34种代谢产物经历了去甲基化、葡萄糖醛酸结合、硫酸结合或混合模式的代谢过程。这是首次对SD大鼠体内CRCD的代谢轮廓进行研究，可为化橘红及其制剂的进一步研究奠定基础。
